2. Academic Validation
  3. Paris Polyphylla Inhibits Colorectal Cancer Cells via Inducing Autophagy and Enhancing the Efficacy of Chemotherapeutic Drug Doxorubicin

Paris Polyphylla Inhibits Colorectal Cancer Cells via Inducing Autophagy and Enhancing the Efficacy of Chemotherapeutic Drug Doxorubicin

  • Molecules. 2019 Jun 3;24(11):2102. doi: 10.3390/molecules24112102.
Liang-Tzung Lin 1 2,  Wu-Ching Uen 3 4,  Chen-Yen Choong 5,  Yeu-Ching Shi 6,  Bao-Hong Lee 7,  Cheng-Jeng Tai 8 9,  Chen-Jei Tai 10 11 12
Affiliations

Affiliations

  • 1 Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11042, Taiwan. [email protected].
  • 2 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 11042, Taiwan. [email protected].
  • 3 School of Medicine, Fujen Catholic University, New Taipei City 24205, Taiwan. [email protected].
  • 4 Department of Hematology and Oncology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei 11042, Taiwan. [email protected].
  • 5 Division of Hematology and Oncology, Department of Internal Medicine, Taipei Medicine University Hospital, Taipei 11042, Taiwan. [email protected].
  • 6 Division of Hematology and Oncology, Department of Internal Medicine, Taipei Medicine University Hospital, Taipei 11042, Taiwan. [email protected].
  • 7 Division of Hematology and Oncology, Department of Internal Medicine, Taipei Medicine University Hospital, Taipei 11042, Taiwan. [email protected].
  • 8 Division of Hematology and Oncology, Department of Internal Medicine, Taipei Medicine University Hospital, Taipei 11042, Taiwan. [email protected].
  • 9 Division of Hematology and Oncology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11042, Taiwan. [email protected].
  • 10 Department of Chinese Medicine, Taipei University Hospital, Taipei 11042, Taiwan. [email protected].
  • 11 Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, Taipei 11042, Taiwan. [email protected].
  • 12 Department of Obstetrics and Gynecology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11042, Taiwan. [email protected].
PMID: 31163662 DOI: 10.3390/molecules24112102
Abstract

Colorectal Cancer is one of the most common cancers worldwide and chemotherapy is the main approach for the treatment of advanced and recurrent cases. Developing an effective complementary therapy could help to improve tumor suppression efficiency and control adverse effects from chemotherapy. Paris polyphylla is a folk medicine for treating various forms of Cancer, but its effect on colorectal Cancer is largely unexplored. The aim of the present study is to investigate the tumor suppression efficacy and the mechanism of action of the ethanolic extract from P. polyphylla (EEPP) in DLD-1 human colorectal carcinoma cells and to evaluate its combined effect with chemotherapeutic drug doxorubicin. The data indicated that EEPP induced DLD-1 cell death via the upregulation of the Autophagy markers, without triggering p53- and caspase-3-dependent Apoptosis. Moreover, EEPP treatment in combination with doxorubicin enhanced cytotoxicity in these tumor cells. Pennogenin 3-O-beta-chacotrioside and polyphyllin VI were isolated from EEPP and identified as the main candidate active components. Our results suggest that EEPP deserves further evaluation for development as complementary chemotherapy for colorectal Cancer.

Keywords

DLD-1 cells; chemotherapy; doxorubicin; drug resistance; folk medicine.

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