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  3. Tannic acid exhibits anti-inflammatory effects on formalin-induced paw edema model of inflammation in rats

Tannic acid exhibits anti-inflammatory effects on formalin-induced paw edema model of inflammation in rats

  • Hum Exp Toxicol. 2019 Nov;38(11):1296-1301. doi: 10.1177/0960327119864154.
A Soyocak 1,  H Kurt 2,  D Turgut Cosan 2,  F Saydam 3,  I U Calis 2,  U K Kolac 2,  Z Ozdemir Koroglu 4,  I Degirmenci 5,  F Sahin Mutlu 6,  H V Gunes 2
Affiliations

Affiliations

  • 1 Department of Medical Biology and Genetics, Medical Faculty, Istanbul Aydin University, Istanbul, Turkey.
  • 2 Department of Medical Biology, Medical Faculty, Eskisehir Osmangazi University, Eskisehir, Turkey.
  • 3 Department of Medical Biology, Faculty of Medicine, Recep Tayyip Erdoğan University, Rize, Turkey.
  • 4 Vocational School of Health Services, Eskisehir Osmangazi University, Eskisehir, Turkey.
  • 5 Department of Medical Biology, Medical Faculty, Kütahya Medical Science University, Kütahya, Turkey.
  • 6 Department of Biostatistics and Medical Informatics, Medical Faculty, Eskisehir Osmangazi University, Eskisehir, Turkey.
PMID: 31315458 DOI: 10.1177/0960327119864154
Abstract

The aim of the study was to determine the relationship between anti-inflammatory effects of the natural polyphenolic compound tannic acid (CAS number: 1401-55-4) and myeloperoxidase (MPO) Enzyme activity in paw edema model. Thirty-five female rats were divided into five groups. The paws of rats were injected subcutaneously in the plantar surface with formalin except for the control group. Indomethacin and tannic acid were intraperitoneally administered 1 h after formalin injection. The paws volume was measured by using vernier caliper. MPO Enzyme activity was determined using 4-aminoantipyrine-phenol solution as the substrate for MPO-mediated oxidation by H2O2. About 17% and 13% edema inhibition has detected in the indomethacin-applied group, at the measurements run every other hour right after the treatment. An inhibition of 16% was found at the group treated with 25 mg/kg tannic acid. However, in the group treated with 50 mg/kg tannic acid, 15% and 7% of the edema inhibition was observed. Serum and paw tissue MPO activities were decreased in treated groups with indomethacin and tannic acid according to formalin control group. Our study results suggest that tannic acid may contribute to the treatment of inflammation by decreasing MPO Enzyme activity, but the molecular mechanism is still not clear.

Keywords

% inhibition; Tannic acid; anti-inflammatory efficiency; formalin; myeloperoxidase; paw edema; rat.

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