Clavulanic acid
Based on 9 publication(s) in Google Scholar
Clavulanic acid is a naturally occurring powerful bacterial β-lactamases inhibitor for research of infections caused by bacteria, including infections of the ears. Clavulanic acid is active against a wide spectrum of gram-positive and gram-negative bacterias.
For research use only. We do not sell to patients.
- Purity: 99.45%
- CAS No.: 58001-44-8
- Formula: C8H9NO5
- Molecular Weight:199.16
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Clavulanic acid
More- Nat Commun. 2022 Mar 2;13(1):1116. [Abstract]
- Virulence. 2026 Dec 31;17(1):2646808. [Abstract]
- Int J Mol Sci. 2023 Oct 27;24(21):15657. [Abstract]
- Eur J Pharmacol. 2024 Dec 5:984:177017. [Abstract]
- Antimicrob Agents Chemother. 2026 Jun 3;70(6):e0126725. [Abstract]
- J Antimicrob Chemother. 2025 Nov 5:dkaf408. [Abstract]
- Genomics. 2022 Nov;114(6):110527. [Abstract]
- Biomed Res Int. 2018 Jul 2:2018:3579832. [Abstract]
- bioRxiv. 2024 May 10.
Customer Validation & Images
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RT-PCR
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IF
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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WB
All Beta-lactamase Isoforms
MoreAll Antibiotic Isoforms
More
Biological Activity
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β-lactam |
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Cell Line
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Type | Value | Description | References |
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| Sf21 | IC50 |
>1000 μM
Compound: Clavulanate
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Inhibition of human BSEP expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake
Inhibition of human BSEP expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake
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[PMID: 21965623] |
| Sf21 | IC50 |
>1000 μM
Compound: Clavulanate
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Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake
Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake
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[PMID: 21965623] |
Clavulanic acid shows a synergistic antibacterial action (against β-lactamase-producing organisms) with Ampicillin[2].
Clavulanic acid inhibits Ab11 and Ab51 strain with MICs of 2-8 μg/mL[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Clavulanic acid (13 mg/kg, i.p.) shows a t1/2 of 6.69 h, AUC of 4.03 mg·h/L in Ab51 infected C57BL/6 mice pneumonia model[3].
Clavulanic acid (100-300 mg/kg, i.p.) shows anti-inflammatory effects on Carrageenan (HY-125474)-induced paw edema rats model[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 58001-44-8
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Appearance Solid
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Molecular Weight 199.16
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Formula C8H9NO5
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Color Off-white to light yellow
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SMILES
O=C([C@@H](/C(O[C@]1([H])C2)=C/CO)N1C2=O)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (9)
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Journal Impact Factor
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Most Recent
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Nat Commun
Antimicrobial resistance and population genomics of multidrug-resistant Escherichia coli in pig farms in mainland China. [Abstract]2022 Mar 2;13(1):1116. PMID: 35236849 -
Virulence
Antibacterial efficacy and mechanism of the novel antimicrobial peptide lachnospirin-1 against Acinetobacter baumannii. [Abstract]2026 Dec 31;17(1):2646808. PMID: 41838520 -
Int J Mol Sci
Clavulanic Acid Improves Memory Dysfunction and Anxiety Behaviors through Upregulating Glutamatergic Transporters in the Nucleus Accumbens of Mice Repeatedly Exposed to Khat Extract. [Abstract]2023 Oct 27;24(21):15657. PMID: 37958641
Clavulanic acid purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2023 Oct 27;24(21):15657. [Abstract]
Average body weight at baseline and after treatment, by group. Two‑way ANOVA showed no significant differences between groups. n = 8–9 per group. Color dots represent individual data. CA (clavulanic acid, 5 mg/kg, i.p. for 7 days); DHK (dihydrokainic acid).
Clavulanic acid purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2023 Oct 27;24(21):15657. [Abstract]
Post hoc comparisons using Tukey’s test indicated the total time spent in closed arms to be higher in the Khat and Khat‑CA‑DHK (CA: clavulanic acid, 5 mg/kg, i.p. for 7 days) groups as compared with the Control and Khat‑CA groups.
Clavulanic acid purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2023 Oct 27;24(21):15657. [Abstract]
Clavulanic acid (5 mg/kg, i.p. for 7 days) reversed the Khat‑induced decrease in GLT‑1 and xCT expression in mice.
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Eur J Pharmacol
Positive allosteric modulation of glutamate transporter reduces cocaine-induced locomotion and expression of cocaine conditioned place preference in rats. [Abstract]2024 Dec 5:984:177017. PMID: 39349114
Clavulanic acid purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2024 Dec 5:984:177017. [Abstract]
Clavulanic acid (CLAV, 10 mg/kg, injected twice daily for 7 days) did not change GLT-1 mRNA expression in the rat PFC.
Clavulanic acid purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2024 Dec 5:984:177017. [Abstract]
Clavulanic acid (CLAV, 10 mg/kg, injected twice daily for 7 days) increased expression of GLT-1 protein in the rat PFC.
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Antimicrob Agents Chemother
Improved hydrolysis of piperacillin by OXA-48-like R214G variants, a selective advantage under piperacillin-tazobactam exposure. [Abstract]2026 Jun 3;70(6):e0126725. PMID: 42053451 -
J Antimicrob Chemother
Unravelling the triad of penicillin-binding proteins, β-lactamase activity, and mRNA dynamics in Pseudomonas aeruginosa AmpC induction. [Abstract]2025 Nov 5:dkaf408. PMID: 41206063 -
Genomics
LRRK2 deficiency mitigates colitis progression by favoring resolution of inflammation and restoring homeostasis of gut microbiota. [Abstract]2022 Nov;114(6):110527. PMID: 36455749 -
Biomed Res Int
In Vitro Activity of β-Lactams in Combination with β-Lactamase Inhibitors against Mycobacterium tuberculosis Clinical Isolates. [Abstract]2018 Jul 2:2018:3579832. PMID: 30065936 -
Solvent & Solubility
DMSO : 13.33 mg/mL (66.93 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 7 mg/mL (35.15 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.33 mg/mL (6.68 mM); Clear solution
This protocol yields a clear solution of ≥ 1.33 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (13.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.33 mg/mL (6.68 mM); Clear solution
This protocol yields a clear solution of ≥ 1.33 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (13.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Parag S Saudagar, et al. Clavulanic acid: a review. Biotechnol Adv. Jul-Aug 2008;26(4):335-51 [Content Brief]
[2]. Neu HC, et al. Clavulanic acid, a novel inhibitor of beta-lactamases. Antimicrob Agents Chemother. 1978 Nov;14(5):650-5. [Content Brief]
[3]. Beceiro A,et al. In vitro activity and in vivo efficacy of clavulanic acid against Acinetobacter baumannii. Antimicrob Agents Chemother. 2009 Oct;53(10):4298-304. [Content Brief]
[4]. Soyocak A, et al. Tannic acid exhibits anti-inflammatory effects on formalin-induced paw edema model of inflammation in rats. Hum Exp Toxicol. 2019 Nov;38(11):1296-1301. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 5.0211 mL | 25.1054 mL | 50.2109 mL | 125.5272 mL |
| 5 mM | 1.0042 mL | 5.0211 mL | 10.0422 mL | 25.1054 mL | |
| 10 mM | 0.5021 mL | 2.5105 mL | 5.0211 mL | 12.5527 mL | |
| 15 mM | 0.3347 mL | 1.6737 mL | 3.3474 mL | 8.3685 mL | |
| 20 mM | 0.2511 mL | 1.2553 mL | 2.5105 mL | 6.2764 mL | |
| 25 mM | 0.2008 mL | 1.0042 mL | 2.0084 mL | 5.0211 mL | |
| 30 mM | 0.1674 mL | 0.8368 mL | 1.6737 mL | 4.1842 mL | |
| DMSO | 40 mM | 0.1255 mL | 0.6276 mL | 1.2553 mL | 3.1382 mL |
| 50 mM | 0.1004 mL | 0.5021 mL | 1.0042 mL | 2.5105 mL | |
| 60 mM | 0.0837 mL | 0.4184 mL | 0.8368 mL | 2.0921 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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