1. Academic Validation
  2. Identification and biological evaluation of natural product Biochanin A

Identification and biological evaluation of natural product Biochanin A

  • Bioorg Chem. 2020 Apr;97:103674. doi: 10.1016/j.bioorg.2020.103674.
Lei Wang 1 Lingzhao Li 1 Quanxiang Han 1 Xiaofang Wang 2 Di Zhao 3 Junqi Liu 4
Affiliations

Affiliations

  • 1 Department of Radiation Oncology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, China.
  • 2 Henan Provincial People's Hospital, Henan Reproductive Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, China.
  • 3 Department of Radiation Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
  • 4 Department of Radiation Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: [email protected].
Abstract

Natural Products have shown promise for epigenetic modulations and thus are therapeutically potential for Cancer prevention and treatment. In this work, we report the identification of natural product Biochanin A as a new LSD1 inhibitor and further biological evaluation in gastric MGC-803 cells. Biochanin A effectively and reversibly inhibited LSD1 (IC50 = 2.95 μM) and showed selective inhibition to LSD1 over MAO-A/B. In gastric MGC-803 cells, Biochanin A induced accumulation of H3K4me1/2, inhibited cell growth moderately (IC50 = 6.77 µM), but was less toxic to normal GES-1 (IC50 > 32 µM). Mechanistic studies showed that Biochanin A suppressed colony formation, cell Apoptosis, and migration of MGC-803 cells. This study may help to elucidate the Anticancer mechanisms of Biochanin A.

Keywords

Biochanin A; Cancer therapy; LSD1 inhibitor; Natural products.

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