2. Academic Validation
  3. Wound healing of nanofiber comprising Polygalacturonic/Hyaluronic acid embedded silver nanoparticles: In-vitro and in-vivo studies

Wound healing of nanofiber comprising Polygalacturonic/Hyaluronic acid embedded silver nanoparticles: In-vitro and in-vivo studies

  • Carbohydr Polym. 2020 Jun 15;238:116175. doi: 10.1016/j.carbpol.2020.116175.
M R El-Aassar 1,  Omar M Ibrahim 2,  Moustafa M G Fouda 3,  Nagham G El-Beheri 4,  Mona M Agwa 5
Affiliations

Affiliations

  • 1 Department of Chemistry, College of Science, Jouf University, Sakaka 2014, Saudi Arabia; Polymer Materials Research Department, Advanced Technology and New Material Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Universities and Research Institutes District, Alexandria 21934, Egypt. Electronic address: [email protected].
  • 2 Polymer Materials Research Department, Advanced Technology and New Material Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Universities and Research Institutes District, Alexandria 21934, Egypt; Department of Medicine and Translational Research, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA.
  • 3 Pre-Treatment and Finishing of Cellulosic-based Fibers Department, Textile Industries Research Division, National Research Center, 33 El- Behooth St, Dokki, Giza, 12311, Egypt. Electronic address: [email protected].
  • 4 Polymer Materials Research Department, Advanced Technology and New Material Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Universities and Research Institutes District, Alexandria 21934, Egypt.
  • 5 Department of Chemistry of Natural and Microbial Products, Pharmaceutical and Drug Industries Research Division, National Research Centre, 33 El- Behooth St, Dokki, Giza, 12311, Egypt.
PMID: 32299548 DOI: 10.1016/j.carbpol.2020.116175
Abstract

The current study is pertaining to develop a novel wound dressing, comprising natural biologically absorbable Materials for wound healing In-vivo. Wound dressing is composed of Polygalacturonic acid, Hyaluronic acid embedded silver nanoparticles, which is further fabricated to form nanofibrous mat, using electrospinning. Silver nanoparticles was prepared using PGA. AgNPs in this formula will serve as an antioxidant and anti-inflammatory that protect cells from destructive effect of elevated ROS and accelerate wound healing. The physical performance and water contact angle for nanofiber was evaluated. The produced nanofiber was characterized by Fourier-transform infrared (FTIR), scanning electron microscopy and thermal analysis. Also, the embedded AgNPs was also characterized by UV-vis spectroscopy and TEM. The nanofiber mates embedded AgNPs was applied to the wounded site of albino rats in-vivo. Histopathological assessment for the wound was fully performed. Also, the antimicrobial activity for the fabricated wound dressing was evaluated against gram+ve and gram -ve bacteria.

Keywords

Antibacterial; Hyaluronic acid; Nanofiber; Polygalacturonic acid; Silver nanoparticles; Wound dressing.

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