2. Academic Validation
  3. Characterization of the sortase A from Lactobacillus acidophilus ATCC 4356 involved in adherence to intestinal cells

Characterization of the sortase A from Lactobacillus acidophilus ATCC 4356 involved in adherence to intestinal cells

  • Future Microbiol. 2020 May;15:485-496. doi: 10.2217/fmb-2019-0219.
Zhen Wu 1,  Jing Wu 1,  Fengxuan Lang 1,  Zhendong Cai 1,  Xiaoqun Zeng 1,  Yuxing Guo 2,  Xiaotao Liu 3,  Daodong Pan 1 2
Affiliations

Affiliations

  • 1 Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang, College of Food & Pharmaceutical Sciences, Ningbo University, Ningbo, Zhejiang, PR China.
  • 2 National R&D Center for Freshwater Fish Processing, Jiangxi Normal University, Nanchang, Jiangxi 330022, PR China.
  • 3 Ningbo Dairy Group, Ningbo, 315211, Zhejiang, China.
PMID: 32476478 DOI: 10.2217/fmb-2019-0219
Abstract

Aim: Confirmation of the enzymatic activity of Class A sortase (SrtA) in probiotic strain Lactobacillus acidophilus associated with the adhesion properties. Materials & methods: SrtA from L. acidophilus ATCC 4356 was purified and its enzymatic properties was investigated by site-directed mutagenesis approach and the sensitivity to metal ions was also detected. Results: SrtA of L. acidophilus ATCC4356 can recognize LPxTG and LPxTD sorting motifs. The active sites of SrtA include His137, Cys198 and Arg205. Furthermore, acacetin can increase the activity of SrtA, while phenyl vinyl sulfone could effectively inhibit the activity of SrtA with an IC50 of 143.32 μg/ml. The adhesion ability of L. acidophilus was also decreased resulting from the inhibition of SrtA activity. Conclusion: The unique properties of SrtA of L. acidophilus can provide some insights into the development of high-adhesion Lactobacillus strains in the GI tract.

Keywords

L. acidophilus; SrtA; adhesion ability; sortase inhibitor.

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