2. Academic Validation
  3. Vitamin C alleviates the senescence of periodontal ligament stem cells through inhibition of Notch3 during long-term culture

Vitamin C alleviates the senescence of periodontal ligament stem cells through inhibition of Notch3 during long-term culture

  • J Cell Physiol. 2021 Feb;236(2):1237-1251. doi: 10.1002/jcp.29930.
Yan Yang 1 2 3 4,  Tao Wang 1 2 3 4,  Sicheng Zhang 1 2 3 4,  Sixun Jia 1 2 3 4,  Hong Chen 1 2 3 4,  Yufeng Duan 1 2 3 4,  Shikai Wang 1 2 3 4,  Guoqing Chen 2 5,  Weidong Tian 1 2 3 4
Affiliations

Affiliations

  • 1 State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
  • 2 National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
  • 3 National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
  • 4 Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
  • 5 School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.
PMID: 32662081 DOI: 10.1002/jcp.29930
Abstract

Periodontal ligament stem cells (PDLSCs), as potential "seed cells" for periodontal tissue repair and regeneration, require to be expanded in vitro for a large scale. Senescence of PDLSCs occurred during long-term culture may compromise the therapeutic effects of PDLSCs. Medium supplements may be useful in antisenescence. However, the effects and mechanisms of vitamin C (Vc) treatment on PDLSCs during long-term culture are still unclear. In this study, we identified that Vc-treated PDLSCs cells maintained a slender morphology, higher growth rate and migration capacity, stemness, and osteogenic differentiation capability during a long-term culture. Moreover, we also identified that Notch3 was significantly upregulated during the cell senescence, and Vc treatment alleviated the senescence of PDLSCs through inhibition of Notch3 during long-term culture. In summary, Vc treatment suppressed PDLSCs senescence by reducing the expression of Notch3 and might be a simple and useful strategy to inhibit cellular senescence during the cell long-term culture.

Keywords

Notch3; cellular senescence; long-term culture; periodontal ligament stem cells; vitamin C.

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