1. Academic Validation
  2. Kinesin-7 CENP-E regulates cell division, gastrulation and organogenesis in development

Kinesin-7 CENP-E regulates cell division, gastrulation and organogenesis in development

  • Eur J Cell Biol. 2020 Aug;99(6):151107. doi: 10.1016/j.ejcb.2020.151107.
Kai-Wei Yu 1 Zhen-Yu She 2 Ya-Lan Wei 3 Ning Zhong 1
Affiliations

Affiliations

  • 1 Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350122, China; Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, Fuzhou, Fujian, 350122, China.
  • 2 Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350122, China; Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, Fuzhou, Fujian, 350122, China. Electronic address: [email protected].
  • 3 Fujian Obstetrics and Gynecology Hospital, Fuzhou, Fujian, 350011, China; Medical Research Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, 350001, China.
Abstract

Kinesin-7 CENP-E motor protein is essential for chromosome alignment and kinetochore-microtubule attachment in cell division. Human CENP-E has recently identified to be linked with the microcephalic primordial dwarfism syndromes associated with a smaller head, brain malformations and a prominent nose. However, the roles of CENP-E in embryonic development remain largely unknown. In this study, we find that zebrafish CENP-E inhibition results in defects in early zygote cleavage, including asymmetric cell division, cell cycle arrest and the developmental abnormalities. We also demonstrate that CENP-E ablation in cultured cells leads to chromosome misalignment, spindle abnormalities and interruptions of the cell cycle. These observations suggest that CENP-E plays a key role in early cell division and cell cycle progression. Furthermore, we also find that CENP-E inhibition results in the defects in the epiboly, the developmental arrest, the smaller head and the abnormal embryo during zebrafish embryogenesis. Our data demonstrate new functions of CENP-E in development and provide insights into its essential roles in organogenesis.

Keywords

CENP-E; Cell division; Chromosome alignment; Genesis; Kinesin-7; Mitosis; Organ.

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