1. Academic Validation
  2. Knockdown of hsa_circ_0037658 inhibits the progression of osteoarthritis via inducing autophagy

Knockdown of hsa_circ_0037658 inhibits the progression of osteoarthritis via inducing autophagy

  • Hum Cell. 2021 Jan;34(1):76-85. doi: 10.1007/s13577-020-00440-9.
Cong Sui 1 Debao Liu 1 Yukang Que 1 Shenglin Xu 1 Yong Hu 2
Affiliations

Affiliations

  • 1 Department of Orthopaedics, The First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei, 230022, Anhui, China.
  • 2 Department of Orthopaedics, The First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei, 230022, Anhui, China. [email protected].
Abstract

Osteoarthritis (OA) is a chronic musculoskeletal degeneration disease that can result in chronic pain and functional disability. Circular RNAs (CirRNAs) are known to be involved in OA. It was reported that hsa_circ_0037658 was notably upregulated in OA tissues; however, the biological role of hsa_circ_0037658 in OA remains unclear. To investigate the function of hsa_circ_0037658 in OA, CHON-001 cells were treated with IL-1β. The effect of hsa_circ_0037658 knockdown on cell growth was tested by CCK-8 and immunofluorescence staining. In addition, the correlation between hsa_circ_0037658 and Autophagy was explored by LC3 staining and western blot. The results indicated that hsa_circ_0037658 was significantly upregulated in IL-1β-treated CHON-001 cells. The silencing of hsa_circ_0037658 could protect CHON-001 cell injury against IL-1β. Moreover, hsa_circ_0037658 shRNA reversed IL-1β-induced cell growth inhibition via inducing cell Autophagy. Furthermore, knockdown of hsa_circ_0037658 notably alleviated the symptom of OA in vivo. To sum up, knockdown of hsa_circ_0037658 suppressed the progression of OA via inducing Autophagy. Thus, hsa_circ_0037658 might serve as a potential target for the treatment of OA.

Keywords

Autophagy; OA; hsa_circ_0037658.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-19312
    99.91%, Autophagy/PI3K Inhibitor