2. Academic Validation
  3. Sodium Danshensu Inhibits Oral Cancer Cell Migration and Invasion by Modulating p38 Signaling Pathway

Sodium Danshensu Inhibits Oral Cancer Cell Migration and Invasion by Modulating p38 Signaling Pathway

  • Front Endocrinol (Lausanne). 2020 Sep 30:11:568436. doi: 10.3389/fendo.2020.568436.
V Bharath Kumar 1,  Shu-Hui Lin 2 3,  B Mahalakshmi 4,  Yu-Sheng Lo 5,  Chia-Chieh Lin 5,  Yi-Ching Chuang 5,  Ming-Ju Hsieh 5 6 7 8,  Mu-Kuan Chen 9
Affiliations

Affiliations

  • 1 Department of Medical Laboratory Science and Biotechnology, Asia University, Taichung, Taiwan.
  • 2 Department of Surgical Pathology, Changhua Christian Hospital, Changhua, Taiwan.
  • 3 Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung, Taiwan.
  • 4 Institute of Research and Development, Duy Tan University, Da Nang, Vietnam.
  • 5 Oral Cancer Research Center, Changhua Christian Hospital, Changhua, Taiwan.
  • 6 Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
  • 7 Department of Holistic Wellness, Mingdao University, Changhua, Taiwan.
  • 8 Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan.
  • 9 Department of Otorhinolaryngology, Head and Neck Surgery, Changhua Christian Hospital, Changhua, Taiwan.
PMID: 33101201 DOI: 10.3389/fendo.2020.568436
Abstract

Background: Oral squamous cell carcinoma (OSCC) that comprises about 90% of all oral Cancer cases is associated with poor prognosis due to its highly metastatic nature. The majority of OSCC treatment options are related detrimental side-effects. Hypothesis/Purpose: The present study aimed at deciphering the effects of a bioactive phytochemical, sodium danshensu, on human oral Cancer cell metastasis. Methods and Results: The treatment of FaDu and Ca9-22 cells with different doses of sodium danshensu (25, 50, and 100 μM) caused a significant reduction in cellular motility, migration, and invasion, as compared to the untreated cells. This effect was associated with a reduced expression of MMP-2, vimentin and N-Cadherin, together with an enhanced expression of E-cadherin and ZO-1. Further investigation on the molecular mechanism revealed that treatment with sodium danshensu caused significant reduction in p38 phosphorylation; however, phosphorylation of ERK1/2 significantly decreased only in FaDu cells, whereas p-JNK1/2 did not show any alteration. A combination of p38 and JNK1/2 inhibitors with sodium danshensu also reduced the migration in the FaDu and Ca9-22 cell lines. Conclusion: Collectively, the present study findings reveal that sodium danshensu execute anti-metastatic effect by suppressing p38 phosphorylation in human oral Cancer. The study identifies sodium danshensu as a potential natural Anticancer agent that can be used therapeutically to manage highly metastatic OSCC.

Keywords

P38 signaling pathway; invasion; migration; oral squamous cell carcinoma (OSCC); sodium danshensu (Pubchem CID: 23711819).

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