Overexpression of EPDR1 has an antitumorigenic effect on breast cancer in vitro

Background: EPDR1 is widely expressed in Cancer, especially colorectal Cancer. However, the biologic function of EPDR1 in breast Cancer is uncertain.

Methods: The expression profile of EPDR1 was assessed by Gene Expression Profiling Interactive Analysis (GEPIA; gepia.cancer-pku.cn). We constructed EPDR1-overexpressing (EPDR1-Ov) plasmids that were transfected into breast Cancer cells (MCF-7 and MDA-MB-453) to examine the EPDR1 effect on their malignant behavior. The EPDR1 overexpression and the critical components of the P53 signaling pathway were determined by western blot or RT-PCR. Cell proliferation, colony formation, invasive capacity, and cell apoptotic proportions were examined after transfection.

Results: mRNA expression of EPDR1 was significantly lessened in breast Cancer tissues when compared to the adjacent normal tissues by data analysis from GEPIA. There was an impairment in proliferative ability, viability, invasion, and anti-apoptotic effect in EPDR1 overexpressed breast Cancer cells. Mechanistic studies showed that EPDR1 overexpression increased the p53, p21 and Bcl-2 expression while inhibiting Bax expression.

Conclusion: EPDR1 inhibited malignant behaviors and promoted Apoptosis in breast Cancer cells by activation of the p53 signaling pathway.

Breast cancer; EPDR1; P53; invasive capacity; proliferation; viability.