2. Academic Validation
  3. 5-Fluoroorotic acid as a selective agent in yeast molecular genetics

5-Fluoroorotic acid as a selective agent in yeast molecular genetics

  • Methods Enzymol. 1987;154:164-75. doi: 10.1016/0076-6879(87)54076-9.
J D Boeke,  J Trueheart,  G Natsoulis,  G R Fink
PMID: 3323810 DOI: 10.1016/0076-6879(87)54076-9
Abstract

5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.

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