5-Fluoroorotic acid monohydrate
Based on 1 Customer Validation
5-Fluoroorotic acid monohydrate is the monohydrate salt form of 5-Fluoroorotic acid (HY-W016819). 5-Fluoroorotic acid monohydrate is the inhibitor for thymidylate synthase that acts as a selective agent in yeast molecular genetics. 5-Fluoroorotic acid monohydrate exhibits antimalarial activity.
For research use only. We do not sell to patients.
- Purity: 99.39%
- CAS No.: 220141-70-8
- Formula: C5H5FN2O5
- Molecular Weight:192.10
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Storage:
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
5-Fluoroorotic acid monohydrate (1 mg/mL, 4-7 days) inhibits thymidylate synthase, selectively inhibits the growth of Ura+ cells with the presence of UMP synthase, and can be used for screening of Ura- mutated yeast (such as Saccharomyces cerevisiae)[1][3].
5-Fluoroorotic acid monohydrate (1 nM-100 μM, 48 h) exhibits antimalarial activity against Plasmodium falciparum with an IC50 of 6.0 nM. 5-Fluoroorotic acid monohydrate exhibits relatively lower cytotoxicity in mammalian cell HT-1080 with an IC50 of 10 μM[4][5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HT-1080
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Concentration:1 nM-100 μM
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Incubation Time:48 h
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Result:Inhibited cell proliferation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:P. yoelii-infected mouse models[5]
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Dosage:0.2-5 mg/kg
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Administration:ip, every 4 hours for 3 days)
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Result:Eliminated malarial parasites.
Caused weight loss, diarrhea, decreased numbers of leukocytes, and increased mortality (5 mg/kg).
Chemical Information
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CAS No. 220141-70-8
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Appearance Solid
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Molecular Weight 192.10
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Formula C5H5FN2O5
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Color White to light yellow
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SMILES
O=C(C(NC1=O)=C(F)C(N1)=O)O.[H]O[H]
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Synonyms
5-Fluorouracil-6-carboxylic acid Monohydrate; 5-FOA; 5-fluoro OA
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
DMSO : 50 mg/mL (260.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Boeke JD, et, al. 5-Fluoroorotic acid as a selective agent in yeast molecular genetics. Methods Enzymol. 1987; 154: 164-75. [Content Brief]
[2]. Nikolova K, et, al. Studies on the antimycotic activity of 5-fluoroorotic acid. Methods Find Exp Clin Pharmacol. 1987 Feb; 9(2): 85-9. [Content Brief]
[3]. Santoso D, et al., Fluoroorotic acid-selected Nicotiana plumbaginifolia cell lines with a stable thymine starvation phenotype have lost the thymine-regulated transcriptional program. Plant Physiol. 2000 Aug;123(4):1517-24. [Content Brief]
[4]. Rathod PK, et al., Selective activity of 5-fluoroorotic acid against Plasmodium falciparum in vitro. Antimicrob Agents Chemother. 1989 Jul;33(7):1090-4. [Content Brief]
[5]. Gómez ZM, et al., Antimalarial activity of a combination of 5-fluoroorotate and uridine in mice. Antimicrob Agents Chemother. 1990 Jul;34(7):1371-5. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.2056 mL | 26.0281 mL | 52.0562 mL | 130.1405 mL |
| 5 mM | 1.0411 mL | 5.2056 mL | 10.4112 mL | 26.0281 mL | |
| 10 mM | 0.5206 mL | 2.6028 mL | 5.2056 mL | 13.0141 mL | |
| 15 mM | 0.3470 mL | 1.7352 mL | 3.4704 mL | 8.6760 mL | |
| 20 mM | 0.2603 mL | 1.3014 mL | 2.6028 mL | 6.5070 mL | |
| 25 mM | 0.2082 mL | 1.0411 mL | 2.0822 mL | 5.2056 mL | |
| 30 mM | 0.1735 mL | 0.8676 mL | 1.7352 mL | 4.3380 mL | |
| 40 mM | 0.1301 mL | 0.6507 mL | 1.3014 mL | 3.2535 mL | |
| 50 mM | 0.1041 mL | 0.5206 mL | 1.0411 mL | 2.6028 mL | |
| 60 mM | 0.0868 mL | 0.4338 mL | 0.8676 mL | 2.1690 mL | |
| 80 mM | 0.0651 mL | 0.3254 mL | 0.6507 mL | 1.6268 mL | |
| 100 mM | 0.0521 mL | 0.2603 mL | 0.5206 mL | 1.3014 mL |
5-Fluoroorotic acid monohydrate Related Classifications
HY-W016819B Related Classifications
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