2. Academic Validation
  3. Expression, purification and microscopic characterization of human ATP-binding cassette sub-family B member 7 protein

Expression, purification and microscopic characterization of human ATP-binding cassette sub-family B member 7 protein

  • Protein Expr Purif. 2021 Jul:183:105860. doi: 10.1016/j.pep.2021.105860.
Zhenzhen Nie 1,  Qinqin Yan 1,  Yuequan Shen 2,  Xue Yang 3
Affiliations

Affiliations

  • 1 State Key Laboratory of Medicinal Chemical Biology and College of Life Sciences, Nankai University, Tianjin, 300350, China.
  • 2 State Key Laboratory of Medicinal Chemical Biology and College of Life Sciences, Nankai University, Tianjin, 300350, China; Synergetic Innovation Center of Chemical Science and Engineering, Tianjin, 300071, China.
  • 3 State Key Laboratory of Medicinal Chemical Biology and College of Life Sciences, Nankai University, Tianjin, 300350, China. Electronic address: [email protected].
PMID: 33689857 DOI: 10.1016/j.pep.2021.105860
Abstract

The ATP-binding cassette sub-family B member 7 (ABCB7) is a membrane transport protein located on the inner membrane of mitochondria, which could be involved in the transport of heme from the mitochondria to the cytosol. ABCB7 also plays a central role in the maturation of cytosolic iron-sulfur (Fe/S) cluster-containing proteins, and mutations can cause a series of mitochondrial defects. X-linked sideroblastic anemia and ataxia (XLSA-A) is a rare cause of early onset ataxia, which may be overlooked due to the usually mild asymptomatic anemia. The genetic defect has been identified as a mutation in the ABCB7 gene at Xq12-q13. Here, we report the expression, purification and the 2D projections derived from negatively stained electron micrographs of recombinant H. sapiens ABCB7 (hABCB7), paving the way from an atomic structure determination of ABCB7.

Keywords

2D classification; ABCB7; Electron microscopy; Expression; Purification.

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