2. Academic Validation
  3. Inhibition of TNFR1 Attenuates LPS Induced Apoptosis and Inflammation in Human Nucleus Pulposus Cells by Regulating the NF-KB and MAPK Signalling Pathway

Inhibition of TNFR1 Attenuates LPS Induced Apoptosis and Inflammation in Human Nucleus Pulposus Cells by Regulating the NF-KB and MAPK Signalling Pathway

  • Neurochem Res. 2021 Jun;46(6):1390-1399. doi: 10.1007/s11064-021-03278-1.
Feng Lv # 1,  Longbiao Yang # 2,  Jianxiu Wang 3,  Zhixiang Chen 2,  Qizhao Sun 2,  Peiguo Zhang 3,  Chentong Guan 3,  Yanbin Liu 4
Affiliations

Affiliations

  • 1 Department of Pain, Zibo Central Hospital, No. 54 The communist youth league road, Zibo, 255000, Shandong Province, People's Republic of China. [email protected].
  • 2 Department of Orthopedics, ShanDong Energy ZiBo Mining Group Co.LTD. Central Hospital, Zibo, 255120, Shandong, People's Republic of China.
  • 3 Department of Pain, Zibo Central Hospital, No. 54 The communist youth league road, Zibo, 255000, Shandong Province, People's Republic of China.
  • 4 Department of Orthopedic Surgery, Liaocheng People's Hospital, Shandong First Medical University, 67 Dong Chang Xi Road, Liaocheng, 252000, Shandong, People's Republic of China. [email protected].
  • # Contributed equally.
PMID: 33713325 DOI: 10.1007/s11064-021-03278-1
Abstract

Intervertebral disc degeneration (IDD) is accompanied by nucleus pulposus (NP) cell Apoptosis, inflammation, and extracellular matrix degradation. Tumour necrosis factor receptor 1 (TNFR1) is a receptor of TNF-α, and is deeply involved in the processes of IDD. However, the effect of TNFR1 inhibition on IDD is not clear. Herein, we report that TNFR1 was increased in LPS-treated HNPCs. The aim of this study was to investigate the potential therapeutic effect of TNFR1 siRNA and selective antagonists of TNFR1 (GSK1995057) on HNPC damage. The results showed that the blockade of TNFR1 by TNFR1 siRNA and GSK1995057 effectively suppressed the cell viability loss, Apoptosis, and inflammation induced by LPS in HNPCs. Furthermore, we found that TNFR1 siRNA and GSK1995057 inhibited activation of the NF-KB and MAPK signalling pathways in LPS-stimulated HNPCs. In summary, the blockade of TNFR1 effectively suppressed LPS-induced Apoptosis and inflammation in HNPCs through the NF-KB and MAPK signalling pathways. This revealed that the blockade of TNFR1 may provide a potential therapeutic treatment for IDD.

Keywords

Intervertebral disc degeneration; Nucleus pulposus cells; TNF-α; TNFR1.

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