2. Academic Validation
  3. Fluorescent labeling affected the structural/conformational properties of arabinoxylans

Fluorescent labeling affected the structural/conformational properties of arabinoxylans

  • Carbohydr Polym. 2021 Aug 1:265:118064. doi: 10.1016/j.carbpol.2021.118064.
Yan Liu 1,  Meng Xiao 2,  Juan Zhao 3,  Xiumin Zhang 4,  Xinzhong Hu 5,  H Douglas Goff 6,  Qingbin Guo 7
Affiliations

Affiliations

  • 1 State Key Laboratory of Food Nutrition and Safety, College of Food Science and Technology, Tianjin University of Science and Technology, Tianjin, 300457, China. Electronic address: [email protected].
  • 2 State Key Laboratory of Food Nutrition and Safety, College of Food Science and Technology, Tianjin University of Science and Technology, Tianjin, 300457, China. Electronic address: [email protected].
  • 3 Research Centre of Modern Analysis Technology, Tianjin University of Science and Technology, Tianjin, 300457, China. Electronic address: [email protected].
  • 4 State Key Laboratory of Food Nutrition and Safety, College of Food Science and Technology, Tianjin University of Science and Technology, Tianjin, 300457, China. Electronic address: [email protected].
  • 5 College of Food Engineering & Nutrition Science, Shaanxi Normal University, Shaanxi, 710119, China. Electronic address: [email protected].
  • 6 Department of Food Science, University of Guelph, Guelph, ON N1G 2W1, Canada. Electronic address: [email protected].
  • 7 State Key Laboratory of Food Nutrition and Safety, College of Food Science and Technology, Tianjin University of Science and Technology, Tianjin, 300457, China. Electronic address: [email protected].
PMID: 33966828 DOI: 10.1016/j.carbpol.2021.118064
Abstract

Three fluorescent-labeling methods, including DMSO/DBTD, reductive amination and fluoresceinamine (FA), were applied to label arabinoxylan (AX). The conjugates were named AF, ATF and ACF, respectively. Compared to AX, the water solubility of AF significantly decreased while that of ATF significantly increased (p < 0.05); both showed decreased solubility with increasing degree of substitution (DS). However, AX was not successfully labeled by the FA method due to poor solubility. The structural and conformational properties of the AF and ATF conjugates under different DS were compared. Results showed that fluorochrome (FITC) was successfully grafted onto AX molecule by using the DMSO/DBTD and reductive amination methods. A slight increased Mw was noticed for both AF and ATF. AF existed in a more compact conformation, while the chain conformation of ATF was more elongated at a lower DS (1.15 %) and more compact at a higher DS (1.34 %).

Keywords

Arabinoxylan; Conformation; Fluorescent labeling; Solubility; Structure.

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