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  2. Exosomes derived from LPS-induced MHs cells prompted an inflammatory response in sepsis-induced acute lung injury

Exosomes derived from LPS-induced MHs cells prompted an inflammatory response in sepsis-induced acute lung injury

  • Respir Physiol Neurobiol. 2021 Oct;292:103711. doi: 10.1016/j.resp.2021.103711.
Xintong Sui 1 Wei Liu 2 Zhi Liu 3
Affiliations

Affiliations

  • 1 Emergency Department, First Hospital of China Medical University, Shenyang City, 110001, Liaoning Province, China. Electronic address: [email protected].
  • 2 Emergency Department, First Hospital of China Medical University, Shenyang City, 110001, Liaoning Province, China. Electronic address: [email protected].
  • 3 Emergency Department, First Hospital of China Medical University, Shenyang City, 110001, Liaoning Province, China. Electronic address: [email protected].
Abstract

Exosome is a novel tool with an essential role in cell communication. However, its role in the pathogenesis of sepsis-induced acute lung injury is currently unknown. Here, we first found that lipopolysaccharide (LPS) could up-regulate the expression of pro-inflammatory cytokines and promote exosomes release in the murine alveolar macrophage cell line (MHs cells). Moreover, we found MHs cells derived exosomes also maintain the pro-inflammatory effect after LPS stimulation. Treating with hydrochloride hydrate (GW4869) could dose-dependently downregulated the release of exosomes and inhibited the upregulation of inflammatory cytokines in MHs cells with LPS treatment. Also, we further identified GW4869 administration induced the remission of histopathologic changes, the reduction of pro-inflammatory cytokines in lung tissue, and inhibit serum exosomes release. These results indicate that the downregulation of exosome release by GW4869 might protect lung tissue from LPS induced injury through the suppression of excessive inflammatory responses, suggesting its potential therapeutic effects on sepsis-induced acute lung injury.

Keywords

Exosome; Inflammatory; MHs cells; Sepsis-induced acute lung injury.

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