2. Academic Validation
  3. Suppression of Bta11975, an α-glucosidase, by RNA interference reduces transmission of tomato chlorosis virus by Bemisia tabaci

Suppression of Bta11975, an α-glucosidase, by RNA interference reduces transmission of tomato chlorosis virus by Bemisia tabaci

  • Pest Manag Sci. 2021 Nov;77(11):5294-5303. doi: 10.1002/ps.6572.
DingYiHui Lu 1 2,  Hao Yue 1 2,  LiPing Huang 1 2,  DeYong Zhang 1 2,  ZhanHong Zhang 3,  Zhuo Zhang 2,  Youjun Zhang 4,  Fan Li 5,  Fei Yan 6,  XuGuo Zhou 7,  XiaoBin Shi 1 2,  Yong Liu 1 2
Affiliations

Affiliations

  • 1 Subcollege of Longping, Graduate School of Hunan University, Changsha, China.
  • 2 Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha, China.
  • 3 Institute of Vegetable, Hunan Academy of Agricultural Sciences, Changsha, China.
  • 4 Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
  • 5 College of Plant Protection, Yunnan Agricultural University, Kunming, China.
  • 6 Institute of Plant Virology, Ningbo University, Ningbo, China.
  • 7 Department of Entomology, University of Kentucky, Lexington, KY, USA.
PMID: 34310017 DOI: 10.1002/ps.6572
Abstract

Background: Tomato chlorosis virus (ToCV) is mainly vectored by Bemisia tabaci in China, which has a worldwide distribution, and greatly reduces the yields of tomato and other vegetables. At present, control of ToCV has been focused mainly by the use of insecticides to control whitefly populations. Transcriptome sequencing showed high expression of the B. tabaci Bta11975 gene, an α-glucosidase (AGLU) during ToCV acquisition by whitefly Mediterranean (MED) species. To investigate the role of Bta11975 gene in ToCV acquisition and transmission by B. tabaci MED, we used RNA interference (RNAi) to reduce the expression of the Bta11975 gene.

Results: The relative expression of the Bta11975 gene was correlated with the ToCV content in B. tabaci. The AGLU is highly expressed in primary salivary gland and gut. After the Bta11975 gene was silenced, the gene expression of B. tabaci was reduced and B. tabaci mortality was increased. Besides, ToCV acquisition by B. tabaci at 48 and 72 h AAP was reduced, and ToCV transmission was significantly reduced by 25 or 50 of B. tabaci.

Conclusions: These results indicate that suppression of expression of the Bta11975 gene in B. tabaci MED by RNAi can reduce acquisition and transmission of ToCV by B. tabaci MED.

Keywords

Bemisia tabaci; RNA interference; tomato chlorosis virus; α-glucosidase.

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