Acarbose
Based on 11 publication(s) in Google Scholar
Acarbose (BAY g 5421), antihyperglycemic agent, is an orally active alpha-glucosidase inhibitor (IC50=11 nM). Acarbose can potentiate the hypoglycemic effects of sulfonylureas or insulin.
For research use only. We do not sell to patients.
- Purity: 99.97%
- CAS No.: 56180-94-0
- Formula: C25H43NO18
- Molecular Weight:645.60
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Acarbose
More- Research (Wash D C). 2026 Feb 25:9:1161. [Abstract]
- Aging Cell. 2026 Jan 20;25(2):e70352.
- Antioxidants (Basel). 2026 May 29;15(6):688. [Abstract]
- Int J Mol Sci. 2025 Feb 6;26(3):1381. [Abstract]
- Nat Prod Bioprospect. 2023 Jan 9;13(1):2. [Abstract]
- Front Pharmacol. 2025 Apr 30:16:1573032. [Abstract]
- J Infect Dis. 2025 Nov 12:jiaf567. [Abstract]
- Pest Manag Sci. 2021 Nov;77(11):5294-5303. [Abstract]
- Phytochemistry. 2022 Apr:196:113083. [Abstract]
- Biochem Syst Ecol. 2025 Oct.
- SSRN. 2025 Jul 25.
Customer Validation & Images
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Bio/Physico-chemical Assay
Biological Activity
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Cell Line
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Type | Value | Description | References |
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| Caco-2 | IC50 |
5 μM
Compound: 11
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Inhibition of maltase in human Caco-2 cell model system after 2 hrs
Inhibition of maltase in human Caco-2 cell model system after 2 hrs
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[PMID: 18595718] |
Acarbose (1, 2, and 3 μM) dose- and time-dependently inhibits TNF-α-induced VSMC proliferation and migration. Acarbose (1, 2, and 3 μM) dose-dependently decreases β-galactosidase, Ras expression and increased p-AMPK expression in TNF-α pre-treated A7r5 cells[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Acarbose (2.5 and 5.0 mg/kg) significantly and dose-dependently decreases the intensity of neointimal IL-6, TNF-α, and iNOS staining, and significantly increases the intensity of neointimal p-AMPK staining. Acarbose (2.5 and 5.0 mg/kg) significantly and dose-dependently decreases neointimal Ras and β-galactosidase expression in HCD-fed rabbits without body weight loss[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 56180-94-0
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Appearance Solid
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Molecular Weight 645.60
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Formula C25H43NO18
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Color White to light yellow
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SMILES
OC[C@H]([C@]1(O[C@@H]2[C@H](O)[C@@H](O)[C@@H]([C@H](O2)C)N[C@]3(C=C(CO)[C@H]([C@@H]([C@H]3O)O)O)[H])[H])O[C@@H]([C@@H]([C@H]1O)O)O[C@@]([H])([C@@H]([C@H](C=O)O)O)[C@@H](CO)O
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Synonyms
BAY g 5421
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Structure Classification
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Initial Source
Actinoplanes sp
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (11)
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Journal Impact Factor
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Most Recent
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Research (Wash D C)
Repurposed Acarbose Targets Nidogen-1 to Remodel the Tumor Stroma and Suppress Portal Vein Tumor Thrombus in Hepatocellular Carcinoma. [Abstract]2026 Feb 25:9:1161. PMID: 41756725 -
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Antioxidants (Basel)
Bioactive Extracts and Constituents from Taraxacum mongolicum: Antioxidant, Anti-Inflammatory, Enzyme-Inhibitory, and Molecular Docking Studies. [Abstract]2026 May 29;15(6):688. PMID: 42351994 -
Int J Mol Sci
Discovery of TRPV4-Targeting Small Molecules with Anti-Influenza Effects Through Machine Learning and Experimental Validation. [Abstract]2025 Feb 6;26(3):1381. PMID: 39941149 -
Nat Prod Bioprospect
2023 Jan 9;13(1):2. PMID: 36617588 -
Front Pharmacol
Canagliflozin alleviates progestin resistance by suppressing RARβ/CRABP2 signaling in THRB knockout endometrial cancer cells. [Abstract]2025 Apr 30:16:1573032. PMID: 40371351 -
J Infect Dis
Ampicillin/Sulbactam in Combination with Ceftazidime/Avibactam against Metallo-β-Lactamase-Producing Carbapenem-Resistant Acinetobacter baumannii: A Genomics-Informed Mechanism-Based Model. [Abstract]2025 Nov 12:jiaf567. PMID: 41223187 -
Pest Manag Sci
Suppression of Bta11975, an α-glucosidase, by RNA interference reduces transmission of tomato chlorosis virus by Bemisia tabaci. [Abstract]2021 Nov;77(11):5294-5303. PMID: 34310017
Acarbose purchased from MedChemExpress. Usage Cited in: Pest Manag Sci. 2021 Nov;77(11):5294-5303. [Abstract]
Effects of Acarbose (500-2000 ng/μL) concentration on AGLU activity in Bemisia tabaci MED. Addition of 750 ng/μL of Acarbose to the nutrient solution caused about 50% inhibition of AGLU activity in B. tabaci MED.
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Phytochemistry
2022 Apr:196:113083. PMID: 34999512 -
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Solvent & Solubility
H2O : 100 mg/mL (154.89 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (154.89 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Protocol
Cell viability is determined using the MTT assay. Cells are seeded in 24-well culture plates at a density of 2×104 cells/well, incubated for 48 h, treated with acarbose at varying concentrations (0.5, 1.0, 2.0, 3.0, and 5.0 μM) for 24 h; or pre-treated with TNF-α (20 ng/mL) for either 24 h or 48 h to evaluate the dose-dependent effects of acarbose on VSMC growth and viability, cultured with 0.5 mg/mL MTT at 37°C in a humidified atmosphere of 5% CO2 for another 4 h, and solubilized with isopropanol. The viable cell number varies directly with the concentration of formazan product measured spectrophotometrically at 563 nm.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Twenty-four male New Zealand white rabbits, weighing 2500 g are used. They are individually housed in metal cages in an air-conditioned room (22 ± 2°C, 55 ± 5% humidity), under a 12 h light/12 h dark cycle with free access to food and water. All rabbits are randomLy assigned to four groups of 6 animals each and are fed either standard chow (Group I), high cholesterol diet (HCD; containing 95.7% standard Purina chow + 3% lard oil + 0.5% cholesterol) (Group II), HCD diet and 2.5 mg/kg per day acarbose (Group III), or HCD diet and 5.0 mg/kg per day acarbose (Group IV). At the end of the 25 weeks, all rabbits are sacrificed by exsanguination under deep anesthesia with pentobarbital (30 mg/kg i.v.) injected via the marginal ear vein. Serum is stored at −80°C prior to measurement of serum values. The aortic arch and thoracic aortas are carefully removed to protect the endothelial lining, and are collected and freed of adhering soft tissue.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (287 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Yee HS, et al. A review of the safety and efficacy of acarbose in diabetes mellitus. Pharmacotherapy. 1996;16(5):792-805. [Content Brief]
[2]. Hanefeld M, et al. Acarbose: oral anti-diabetes drug with additional cardiovascular benefits [published correction appears in Expert Rev Cardiovasc Ther. 2009 Mar;7(3):330]. Expert Rev Cardiovasc Ther. 2008;6(2):153-163. [Content Brief]
[3]. Oki T, et al. Evaluation of alpha-glucosidase inhibition by using an immobilized assay system. Biol Pharm Bull. 2000;23(9):1084-1087. [Content Brief]
[4]. Zhang Q, et al. Acarbose Reduces Blood Glucose by Activating miR-10a-5p and miR-664 in Diabetic Rats. PLoS One. 2013 Nov 18;8(11):e79697. [Content Brief]
[5]. Chan KC, et al. Pleiotropic effects of acarbose on atherosclerosis development in rabbits are mediated via upregulating AMPK signals. Sci Rep. 2016 Dec 7;6:3864 [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.5489 mL | 7.7447 mL | 15.4895 mL | 38.7237 mL |
| 5 mM | 0.3098 mL | 1.5489 mL | 3.0979 mL | 7.7447 mL | |
| 10 mM | 0.1549 mL | 0.7745 mL | 1.5489 mL | 3.8724 mL | |
| 15 mM | 0.1033 mL | 0.5163 mL | 1.0326 mL | 2.5816 mL | |
| 20 mM | 0.0774 mL | 0.3872 mL | 0.7745 mL | 1.9362 mL | |
| 25 mM | 0.0620 mL | 0.3098 mL | 0.6196 mL | 1.5489 mL | |
| 30 mM | 0.0516 mL | 0.2582 mL | 0.5163 mL | 1.2908 mL | |
| 40 mM | 0.0387 mL | 0.1936 mL | 0.3872 mL | 0.9681 mL | |
| 50 mM | 0.0310 mL | 0.1549 mL | 0.3098 mL | 0.7745 mL | |
| 60 mM | 0.0258 mL | 0.1291 mL | 0.2582 mL | 0.6454 mL | |
| 80 mM | 0.0194 mL | 0.0968 mL | 0.1936 mL | 0.4840 mL | |
| 100 mM | 0.0155 mL | 0.0774 mL | 0.1549 mL | 0.3872 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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