2. Academic Validation
  3. Glucagon-like peptide-1 attenuated carboxymethyl lysine induced neuronal apoptosis via peroxisome proliferation activated receptor-γ

Glucagon-like peptide-1 attenuated carboxymethyl lysine induced neuronal apoptosis via peroxisome proliferation activated receptor-γ

  • Aging (Albany NY). 2021 Jul 29;13(14):19013-19027. doi: 10.18632/aging.203351.
Haoqiang Zhang 1 2,  Bing Song 3,  Wenwen Zhu 1 2,  Lili Liu 3,  Xiqiao He 3,  Zheng Wang 1 2,  Ke An 1 2,  Wuyou Cao 1 2,  Jijing Shi 1 2,  Shaohua Wang 1 2
Affiliations

Affiliations

  • 1 Department of Endocrinology, Affiliated Zhongda Hospital of Southeast University, Nanjing 210000, Jiangsu Province, China.
  • 2 School of Medicine, Southeast University, Nanjing 210000, Jiangsu Province, China.
  • 3 Department of Endocrinology, First Affiliated Hospital of Jinzhou Medical University, Jinzhou 1210001, Liaoning Province, China.
PMID: 34326274 DOI: 10.18632/aging.203351
Abstract

Backgrounds and aims: The role of peroxisome proliferator activated receptor-γ (PPAR-γ) in neuronal Apoptosis remains unclear. We aim to investigate the role of PPAR-γ in glucagon-like peptide-1 (GLP-1) alleviated neuronal Apoptosis induced by carboxymethyl-lysine (CML).

Materials and methods: In vitro, PC12 cells were treated by CML/GLP-1. Moreover. the function of PPAR-γ was blocked by GW9662. In vivo, streptozotocin (STZ) was used to induce diabetic rats with neuronal Apoptosis. The cognitive function of rats was observed by Morris water maze. Apoptosis was detected by TUNEL assay. Bcl2, Bax, PPAR-γ and receptor of GLP-1 (GLP-1R) were measured by western blotting or immunofluorescence.

Results: In vitro experiment, CML triggered Apoptosis, down-regulated GLP-1R and PPAR-γ. Moreover, GLP-1 not only alleviated the Apoptosis, but also increased levels of PPAR-γ. GW9662 abolished the neuroprotective effect of GLP-1 on PC12 cells from Apoptosis. Furthermore, GLP-1R promoter sequences were detected in the PPAR-γ antibody pulled mixture. GPL-1 levels decreased, while CML levels increased in diabetic rats, compared with control rats. Additionally, we observed elevated Bax, decreased bcl2, GLP-1R and PPAR-γ in diabetic rats.

Conclusions: GLP-1 could attenuate neuronal Apoptosis induced by CML. Additionally, PPAR-γ involves in this process.

Keywords

apoptosis; carboxymethyl-lysine; glucagon-like peptide-1; peroxisome proliferator activated receptor-γ.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-16578
    99.87%, PPARγ Antagonist