2. Academic Validation
  3. Downregulation of Hotair or LSD1 Impaired Heart Regeneration in the Neonatal Mouse

Downregulation of Hotair or LSD1 Impaired Heart Regeneration in the Neonatal Mouse

  • DNA Cell Biol. 2021 Sep;40(9):1177-1184. doi: 10.1089/dna.2021.0095.
Qiaoman Fei 1 2,  Manman Qiu 1,  Guanwei Fan 3,  Bo Zhang 4,  Qin Wang 5,  Sipei Zhang 6,  Shuying Wang 1,  Bing Yang 7,  Ling Zhang 1
Affiliations

Affiliations

  • 1 Department of Physiology, College of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.
  • 2 Precision Medical Institute, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
  • 3 First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China.
  • 4 Department of Immunology, Tianjin Key Laboratory of Cellular and Molecular Immunology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.
  • 5 Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin, China.
  • 6 Department of Pharmacy, Tianjin Chest Hospital, Tianjin, China.
  • 7 Department of Cell Biology, College of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.
PMID: 34432529 DOI: 10.1089/dna.2021.0095
Abstract

Previous studies have shown that lysine-specific demethylase 1 (LSD1) could regulate cell cycle progression through demethylation. The 3'domain of HOX transcript antisense RNA (Hotair) combined with the LSD1/CoREST/REST complex helps LSD1 target the corresponding gene. However, its role in mice's myocardial regeneration is still unclear. The heart from neonatal mice shows strong myocardial regeneration ability, but this ability disappears 7 days after birth. Our study shows that the myocardial tissue highly expresses Hotair and Lsd1 within 1 week after birth, consistent with the myocardial regeneration time window. Knockdown Lsd1 or Hotair expression by RNA interference could inhibit myocardial regeneration and cardiomyocyte proliferation. Our results suggest that Hotair-mediated demethylation of LSD1 may play an important role in myocardial regeneration in neonatal mice.

Keywords

Hotair; LSD1; demethylation; regeneration.

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