Pyrroline-5-Carboxylate Reductase-2 Promotes Colorectal Cancer Progression via Activating PI3K/AKT/mTOR Pathway

Aim: The aim of this study was to investigate the effect and underlying pathway of pyrroline-5-carboxylate reductase-2 (PYCR2) on colorectal Cancer (CRC).

Methods: The Cancer Genome Atlas (TCGA) database was used to analyze PYCR2 expression levels and clinical information. Cell proliferation was evaluated using colony forming and EdU assay. Cell Apoptosis rate was determined using flow cytometry. Cell migration and invasion were measured by performing a Transwell assay, and PYCR2, MMP-2, MMP-9, Bax, cleaved Caspase-3, Bcl-2, cleaved PARP, p-PI3K, PI3K, p-AKT, Akt, p-mTOR, and mTOR protein levels were detected by Western blot.

Results: A review of the TCGA database revealed that PYCR2 was highly expressed in CRC patients and that high PYCR2 expression was associated with advanced stage, adenocarcinoma, nodal metastasis, and poor survival rate. Moreover, PYCR2 knockdown reduced cell viability, proliferation, migration, and invasion and increased Apoptosis. Additionally, PYCR2 knockdown increased Bax, cleaved Caspase-3, and cleaved PARP levels and decreased Bcl-2, MMP-2, MMP-9, p-PI3K, p-AKT, and p-mTOR levels in CRC cells. Effects of silencing PYCR2 on proliferation, migration, invasion, Apoptosis, and the PI3K/Akt/mTOR pathway in CRC cells were all reversed using a PI3K Activator (740Y-P).

Conclusion: PYCR2 was highly expressed in CRC, and its knockdown suppressed CRC tumorigenesis via inhibiting the activation of PI3K/Akt/mTOR pathway. This finding provides a new theoretical foundation for the treatment of CRC.