1. PI3K/Akt/mTOR
    Autophagy
  2. PI3K
    Autophagy
  3. 740 Y-P

740 Y-P (Synonyms: 740YPDGFR; PDGFR 740Y-P)

Cat. No.: HY-P0175 Purity: 99.67%
Handling Instructions

740 Y-P (740YPDGFR; PDGFR 740Y-P) is a potent and cell-permeable PI3K activator. 740 Y-P readily binds GST fusion proteins containing both the N- and C- terminal SH2 domains of p85 but fails to bind GST alone.

For research use only. We do not sell to patients.

740 Y-P Chemical Structure

740 Y-P Chemical Structure

CAS No. : 1236188-16-1

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1 mg USD 120 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 23 publication(s) in Google Scholar

Top Publications Citing Use of Products

    740 Y-P purchased from MCE. Usage Cited in: Acta Biomater. 2018 Nov;81:278-292.

    PC12 cells are pretreated with 740-YP (30 μM), or SC79 (13.7 μM), or 3BDO (100 μM) for 1 h, and for the duration of GO (50 μg/mL) incubation for 24 h. LC3 turnover is detected by western blotting.

    740 Y-P purchased from MCE. Usage Cited in: J Exp Clin Cancer Res. 2020 Jun 30;39(1):123.

    Protein expression levels of p-AktSer473, p-mTORSer2448 with or without 740Y-P treatment are measured by Western blotting. Pretreatment with 740Y-P (PI3K agonist) inhibits downregulation of p-AktSer473 and p-mTORSer2448 expression induced by overexpression of BDH2.
    • Biological Activity

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    Description

    740 Y-P (740YPDGFR; PDGFR 740Y-P) is a potent and cell-permeable PI3K activator. 740 Y-P readily binds GST fusion proteins containing both the N- and C- terminal SH2 domains of p85 but fails to bind GST alone[1].

    IC50 & Target

    PI3K

     

    In Vitro

    740 Y-P (50 μg/mL; 48 hours) specificly stimulates mitogenesis in medium is better than EGF or FGF at stimulating entry into S-phase, it shows the percentage of cells in S-phase for 48.3% in C2 cells. Additionally, LY294002 (HY-10108) or Wortmannin (HY-10197) potently inhibits the mitogenic response stimulated by the peptide[1].
    740 Y-P (1 μg/mL) stimulates mitogenesis at the lowest concentration tested. The peptide stimulates mitogenesis in both the presence and absence of serum (0.5%), and in the former instance a maximal response observed at 50 μg/mL. 740Y-P to stimulate mitogenesis is highly specific and not a general feature of a cell permeable SH2 domain binding peptides[1].
    740 Y-P (30 μM; 24 hours) remarkably inhibits the level of LC3-II/LC3-I in GO-induced PC12 cells[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Western Blot Analysis[2]

    Cell Line: PC12 cells
    Concentration: 30 μM
    Incubation Time: 24 hours
    Result: Inhibited the protein expression of LC3-II.
    In Vivo

    740 Y-P is not only internalised in living cells but can also interact with p85 in vivo[1].
    740 Y-P (intraperitoneal injection; 10 mg/kg; 6 weeks) decreases the degree of ROS levels in Aβ(25-32) treated hippocampal tissues and increases the extent of AKT and PI3K phosphorylation in alzheimer’s disease (AD) rat model[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    3270.70

    Formula

    C₁₄₁H₂₂₂N₄₃O₃₉PS₃

    CAS No.

    1236188-16-1

    Sequence Shortening

    RQIKIWFQNRRMKWKKSDGG-{PO2Y}-MDMS

    SMILES

    [RQIKIWFQNRRMKWKKSDGG-{PO2-Tyr}-MDMS]

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    Protect from light

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    H2O : 2 mg/mL (0.61 mM; Need ultrasonic)

    References

    Purity: 99.67%

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    Keywords:

    740 Y-P740YPDGFR PDGFR 740Y-PPI3KAutophagyPhosphoinositide 3-kinasethoracicaorticVSMCcancerSH2p85 bindingpeptide alzheimer’s diseaseADInhibitorinhibitorinhibit

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    Product Name:
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    Cat. No.:
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