1. PI3K/Akt/mTOR
    Autophagy
  2. PI3K
    Autophagy
  3. 740 Y-P TFA

740 Y-P TFA (Synonyms: 740YPDGFR TFA; PDGFR 740Y-P TFA)

Cat. No.: HY-P0175A
Handling Instructions

740 Y-P TFA is a potent and cell-permeable PI3K activator. 740 Y-P TFA readily binds GST fusion proteins containing both the N- and C- terminal SH2 domains of p85 but fails to bind GST alone.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

740 Y-P TFA Chemical Structure

740 Y-P TFA Chemical Structure

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Top Publications Citing Use of Products

    740 Y-P TFA purchased from MCE. Usage Cited in: Acta Biomater. 2018 Nov;81:278-292.

    PC12 cells are pretreated with 740-YP (30 μM), or SC79 (13.7 μM), or 3BDO (100 μM) for 1 h, and for the duration of GO (50 μg/mL) incubation for 24 h. LC3 turnover is detected by western blotting.

    740 Y-P TFA purchased from MCE. Usage Cited in: J Exp Clin Cancer Res. 2020 Jun 30;39(1):123.

    Protein expression levels of p-AktSer473, p-mTORSer2448 with or without 740Y-P treatment are measured by Western blotting. Pretreatment with 740Y-P (PI3K agonist) inhibits downregulation of p-AktSer473 and p-mTORSer2448 expression induced by overexpression of BDH2.
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    Description

    740 Y-P TFA is a potent and cell-permeable PI3K activator. 740 Y-P TFA readily binds GST fusion proteins containing both the N- and C- terminal SH2 domains of p85 but fails to bind GST alone[1].

    In Vitro

    740 Y-P TFA (50 μg/ml; 48 hours) specificly stimulates mitogenesis in medium is better than EGF or FGF at stimulating entry into S-phase, it shows the percentage of cells in S-phase for 48.3% in C2 cells. Additionally, LY294002 or wortmannin potently inhibits the mitogenic response stimulated by the 740 Y-P TFA peptide[1].
    740 Y-P TFA (1 μg/mL) stimulates mitogenesis at the lowest concentration tested. The peptide stimulates mitogenesis in both the presence and absence of serum (0.5%), and in the former instance a maximal response observed at 50 μg/mL. 740Y-P to stimulate mitogenesis is highly specific and not a general feature of a cell permeable SH2 domain binding peptides[1].
    740 Y-P TFA (30 μM; 24 hours) remarkably inhibits the level of LC3-II/LC3-I in GO-induced PC12 cells[2].

    Western Blot Analysis[2]

    Cell Line: PC12 cells
    Concentration: 30 μM
    Incubation Time: 24 hours
    Result: Inhibited the protein expression of LC3-II.
    Molecular Weight

    3384.72

    Formula

    C₁₄₃H₂₂₃N₄₃F₃O₄₁PS₃

    Sequence

    Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Ser-Asp-Gly-Gly-{PO2-Tyr}-Met-Asp-Met-Ser

    Sequence Shortening

    RQIKIWFQNRRMKWKKSDGG-{PO2Y}-MDMS

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.

    References
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    Keywords:

    740 Y-P740YPDGFRPDGFR 740Y-PPI3KAutophagyPhosphoinositide 3-kinasethoracicaorticVSMCcancerSH2p85 bindingpeptideInhibitorinhibitorinhibit

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