740 Y-P TFA (Synonyms: 740YPDGFR TFA; PDGFR 740Y-P TFA)

Cat. No.: HY-P0175A: Data Sheet Handling Instructions
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740 Y-P TFA is a potent and cell-permeable PI3K activator. 740 Y-P TFA readily binds GST fusion proteins containing both the N- and C- terminal SH2 domains of p85 but fails to bind GST alone.

For research use only. We do not sell to patients.

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Other In-stock Forms of 740 Y-P TFA:

740 Y-P

Other Forms of 740 Y-P TFA:

740 Y-P In-stock

241 Publications Citing Use of MCE 740 Y-P TFA

: 740 Y-P TFA purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Apr;12(13):e2410032. [Abstract]; Protein expression and quantification of SGLT1, GLUT2, p-PI3K, p-AKT, p-S6K1, and p-eIF4B were performed. GAPDH was used to normalize the data. 740Y-P (30 μM; 24 h) was an activator of the PI3K/Akt signaling pathway.

: 740 Y-P TFA purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Jul 8;14(11):4278-4296. [Abstract]; Western blotting was used to measure p-PI3K/PI3K, p-AKT/AKT, and p-mTOR/mTOR expression in MODE-K cells incubated with 740 Y-P (25 μg/mL; 24 h).

: 740 Y-P TFA purchased from MedChemExpress. Usage Cited in: Exp Mol Med. 2023 May;55(5):939-951. [Abstract]; Western blot showing PI3K, AKT, p-AKT, GPX4, ACSL4, TFR1, and SLC7A11 in the tissue samples of ApoE−/− mice infused with saline or Ang II, with or without 740 Y-P (10 mg/kg/d) treatment. n = 3 in each group.

: 740 Y-P TFA purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2020 Jun 30;39(1):123. [Abstract]; Protein expression levels of p-AktSer473, p-mTORSer2448 with or without 740Y-P treatment are measured by Western blotting. Pretreatment with 740Y-P (PI3K agonist) inhibits downregulation of p-AktSer473 and p-mTORSer2448 expression induced by overexpression of BDH2.

: 740 Y-P TFA purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2018 Nov:81:278-292. [Abstract]; PC12 cells are pretreated with 740-YP (30 μM), or SC79 (13.7 μM), or 3BDO (100 μM) for 1 h, and for the duration of GO (50 μg/mL) incubation for 24 h. LC3 turnover is detected by western blotting.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

740 Y-P TFA is a potent and cell-permeable PI3K activator. 740 Y-P TFA readily binds GST fusion proteins containing both the N- and C- terminal SH2 domains of p85 but fails to bind GST alone^[1].

In Vitro

740 Y-P TFA (50 μg/ml; 48 hours) specificly stimulates mitogenesis in medium is better than EGF or FGF at stimulating entry into S-phase, it shows the percentage of cells in S-phase for 48.3% in C2 cells. Additionally, LY294002 or wortmannin potently inhibits the mitogenic response stimulated by the 740 Y-P TFA peptide^[1].
740 Y-P TFA (1 μg/mL) stimulates mitogenesis at the lowest concentration tested. The peptide stimulates mitogenesis in both the presence and absence of serum (0.5%), and in the former instance a maximal response observed at 50 μg/mL. 740Y-P to stimulate mitogenesis is highly specific and not a general feature of a cell permeable SH2 domain binding peptides^[1].
740 Y-P TFA (30 μM; 24 hours) remarkably inhibits the level of LC3-II/LC3-I in GO-induced PC12 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	PC12 cells
Concentration:	30 μM
Incubation Time:	24 hours
Result:	Inhibited the protein expression of LC3-II.

Molecular Weight

3270.70 (free acid)

Formula

C₁₄₁H₂₂₂N₄₃O₃₉PS₃.xC₂HF₃O₂

Sequence

Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Ser-Asp-Gly-Gly-{Tyr(PO2)}-Met-Asp-Met-Ser

Sequence Shortening

RQIKIWFQNRRMKWKKSDGG-{PO2Y}-MDMS

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Derossi D, et al. Stimulation of mitogenesis by a cell-permeable PI 3-kinase binding peptide. [Content Brief]

[2]. Xiaoli Feng, et al. Graphene Oxide Induces p62/SQSTM-dependent Apoptosis Through the Impairment of Autophagic Flux and Lysosomal Dysfunction in PC12 Cells. Acta Biomater. 2018 Nov;81:278-292. [Content Brief]

740 Y-P TFA Related Classifications

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

740 Y-P740YPDGFRPDGFR 740Y-PPI3KAutophagyPhosphoinositide 3-kinasethoracicaorticVSMCcancerSH2p85 bindingpeptideInhibitorinhibitorinhibit

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