1. Academic Validation
  2. miR-186-3p attenuates the tumorigenesis of cervical cancer via targeting insulin-like growth factor 1 to suppress PI3K-Akt signaling pathway

miR-186-3p attenuates the tumorigenesis of cervical cancer via targeting insulin-like growth factor 1 to suppress PI3K-Akt signaling pathway

  • Bioengineered. 2021 Dec;12(1):7079-7092. doi: 10.1080/21655979.2021.1977053.
Xiurong Lu 1 Xiao Song 1 Xiaohui Hao 1 Xiaoyu Liu 1 Xianyu Zhang 1 Na Yuan 1 Huan Ma 1 Zhilin Zhang 1
Affiliations

Affiliation

  • 1 Department of Radiotherapy, The First Affiliated Hospital of Hebei North University, Zhangjiakou, Hebei, China.
Abstract

miR-186-3p acts as a tumor suppressor in various cancers. This study aimed to explore the expression levels of miR-186-3p and its role in cervical Cancer. We analyzed the effects of miR-186-3p and insulin-like growth factor 1 (IGF1) on the proliferation, invasion, and Apoptosis of cervical Cancer cells in vitro by regulating the PI3K/Akt signaling pathway. In cervical Cancer tissues and cells, miR-186-3p was downregulated, and IGF1 was upregulated. In addition, miR-186-3p inhibited cell proliferation and invasion and enhanced Apoptosis of cervical Cancer cells. Moreover, our results showed that miR-186-3p inversely regulated the mRNA expression of IGF1 through direct contact. Knockdown of IGF1 reversed the results of miR-186-3p inhibitor in cervical Cancer cells. In addition, the PI3K/Akt signaling pathway was activated by the miR-186-3p inhibitor, although partially arrested by IGF1 knockdown. The PI3K/Akt signaling pathway inhibitor suppressed miR-186-3p inhibitor-stimulated cell proliferation in cervical Cancer. In conclusion, miR-186-3p inhibits tumorigenesis of cervical Cancer by repressing IGF1, which inactivates the PI3K/Akt signaling pathway, implicating miR-186-3p as a potential new target for the treatment of cervical Cancer.

Keywords

Cervical cancer; IGF1; PI3K/AKT; miR-186-3p; tumorigenesis.

Figures
Products