1. Academic Validation
  2. Selective depletion of hepatic stellate cells-specific LOXL1 alleviates liver fibrosis

Selective depletion of hepatic stellate cells-specific LOXL1 alleviates liver fibrosis

  • FASEB J. 2021 Oct;35(10):e21918. doi: 10.1096/fj.202100374R.
Aiting Yang 1 2 3 Xuzhen Yan 3 4 Hufeng Xu 1 2 3 Xu Fan 3 4 Mengyang Zhang 3 4 Tao Huang 1 2 3 Weiyu Li 3 4 Wei Chen 1 2 3 Jidong Jia 2 3 4 Hong You 2 3 4
Affiliations

Affiliations

  • 1 Experimental and Translational Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing, P.R. China.
  • 2 Beijing Clinical Medicine Institute, Beijing, P.R. China.
  • 3 National Clinical Research Center of Digestive Diseases, Beijing, P.R. China.
  • 4 Liver Research Center, Beijing Key Laboratory of Translational Medicine in Liver Cirrhosis, Beijing Friendship Hospital, Capital Medical University, Beijing, P.R. China.
Abstract

The role of LOXL1 in fibrosis via mediating ECM crosslinking and stabilization is well established; however, the role of hepatic stellate cells (HSCs)-specific LOXL1 in the development of fibrosis remains unknown. We generated HSCs-specific Loxl1-depleted mice (Loxl1Gfap-cre mice) to investigate the HSCs-specific contribution of LOXL1 in the pathogenesis of fibrosis. Loxl1fl/fl mice were used as the control. Furthermore, we used RNA sequencing to explore the underlying changes in the transcriptome. Results of the sirius red staining, type I collagen immunolabeling, and hydroxyproline content analysis, coupled with the reduced expression of profibrogenic genes revealed that Loxl1Gfap-cre mice with CCl4 -induced fibrosis exhibited decreased hepatic fibrosis. In addition, Loxl1Gfap-cre mice exhibited reduced macrophage tissue infiltration by CD68-positive cells and decreased expression of inflammatory genes compared with the controls. RNA sequencing identified Integrin α8 (ITGA8) as a key modulator of LOXL1-mediated liver fibrosis. Functional analyses showed that siRNA silencing of Itga8 in cultured fibroblasts led to a decline in the LOXL1 expression and inhibition of fibroblast activation. Mechanistic analyses indicated that LOXL1 activated the FAK/PI3K/Akt/HIF1a signaling pathway, and the addition of inhibitors of FAK or PI3K reversed these results via downregulation of LOXL1. Furthermore, HIF1a directly interacted with LOXL1 and upregulated its expression, indicating that LOXL1 can positively self-regulate by forming a positive feedback loop with the FAK/PI3K/Akt/HIF1a pathway. We demonstrated that HSCs-specific Loxl1 deficiency prevented fibrosis, inflammation and that ITGA8/FAK/PI3K/Akt/HIF1a was essential for the function and expression of LOXL1. Knowledge of this approach can provide novel mechanisms and targets to treat fibrosis in the future.

Keywords

ITGA8; LOXL1; extracellular matrix; hepatic stellate cells; liver fibrosis.

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