1. Academic Validation
  2. Macrophage polarization in vitro and in vivo modified by contact with fragmented chitosan hydrogel

Macrophage polarization in vitro and in vivo modified by contact with fragmented chitosan hydrogel

  • J Biomed Mater Res A. 2022 Apr;110(4):773-787. doi: 10.1002/jbm.a.37326.
Ysander von Boxberg 1 2 3 Sylvia Soares 1 2 3 Camille Giraudon 1 2 3 Laurent David 4 5 Maud Viallon 4 5 Alexandra Montembault 4 5 Fatiha Nothias 1 2 3
Affiliations

Affiliations

  • 1 Sorbonne Universités, UPMC Paris 06, UM 119, Institut de Biologie Paris Seine (IBPS), Paris, France.
  • 2 CNRS UMR 8246, Neuroscience Paris Seine (NPS), Paris, France.
  • 3 INSERM U 1130, Neuroscience Paris Seine (NPS), Paris, France.
  • 4 Université de Lyon, Université Claude Bernard Lyon-1, Villeurbanne, France.
  • 5 CNRS UMR 5223, Ingénierie des Matériaux Polymères (IMP), Villeurbanne, France.
Abstract

We have previously shown that implantation of a fragmented chitosan hydrogel suspension (chitosan-FPHS) into a traumatic spinal cord lesion in adult rats led to significant axon regrowth and functional recovery, which was associated to a modulation of inflammation. Using an in vitro culture system, we show here that polarization of bone marrow-derived macrophages is indeed modified by direct contact with chitosan-FPHS. Reducing the degree of acetylation (DA) and raising the concentration of chitosan (Cp, from 1.5% to 3%), favors macrophage polarization toward anti-inflammatory subtypes. These latter also migrate and adhere efficiently on low, but not high DA chitosan-FPHS, both in vitro and in vivo, while inflammatory macrophages rarely invade a chitosan-FPHS implant in vivo, no matter the DA. Our in vitro model setup should prove a valuable tool for screening diverse biomaterial formulations and combinations thereof for their inflammatory potential prior to implantation in vivo.

Keywords

acetylation degree; bone marrow macrophages; cell culture; chitosan hydrogel; inflammation; spinal cord injury.

Figures
Products