2. Academic Validation
  3. An aggregation-induced emission platform for efficient Golgi apparatus and endoplasmic reticulum specific imaging

An aggregation-induced emission platform for efficient Golgi apparatus and endoplasmic reticulum specific imaging

  • Chem Sci. 2021 Oct 5;12(41):13949-13957. doi: 10.1039/d1sc03932f.
Peihong Xiao 1 2,  Ke Ma 1 2,  Miaomiao Kang 1 2,  Luyi Huang 3,  Qian Wu 4,  Nan Song 1 2,  Jinyin Ge 1 2,  Dan Li 1 2,  Jianxia Dong 5,  Lei Wang 1,  Dong Wang 1,  Ben Zhong Tang 4 6
Affiliations

Affiliations

  • 1 Center for AIE Research, Shenzhen Key Laboratory of Polymer Science and Technology, Guangdong Research Center for Interfacial Engineering of Functional Materials, College of Materials Science and Engineering, Shenzhen University Shenzhen 518060 China [email protected].
  • 2 Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University Shenzhen 518060 China.
  • 3 Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical University Chongqing 400010 China.
  • 4 Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research, Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong 999077 China [email protected].
  • 5 Department of Clinical Pharmacy, West China Hospital of Sichuan University Chengdu 610041 Sichuan Province China.
  • 6 Shenzhen Institute of Molecular Aggregate Science and Engineering, School of Science and Engineering, The Chinese University of Hong Kong Shenzhen, 2001 Longxiang Boulevard, Longgang District Shenzhen City Guangdong 518172 China [email protected].
PMID: 34760181 DOI: 10.1039/d1sc03932f
Abstract

As two important subcellular organelles in eukaryotic cells, the Golgi apparatus (GA) and endoplasmic reticulum (ER) have recently captivated much interest due to their considerable importance in many biofunctions and role as critical biomarkers for various diseases. The development of efficient GA- and ER-specific probes is of great significance, but remains an appealing yet significantly challenging task. Herein, we reported for the first time the construction of an aggregation-induced emission (AIE) platform for GA and ER fluorescent probes, termed as AIE-GA and AIE-ER, by facile synthesis and simple functionalization. Their excellent targeting specificity to GA or ER, remarkable photostability, high brightness, and low working concentration make AIE-GA and AIE-ER significantly impressive and superior to commercially available probes. Moreover, molecular docking calculations are performed to validate the targeting mechanism of the two AIE probes.

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