Chlorobisphenol A activated kisspeptin/GPR54-GnRH neuroendocrine signals through ERα and GPER pathway in neuronal GT1-7 cells

Chlorobisphenol A (Cl_xBPA) is a kind of novel estrogenic compounds. The present study aims to investigate the effects of three Cl_xBPA compounds on the kisspeptin/G protein-coupled receptor 54 (GPR54, also named KissR1)-gonadotropin-releasing hormone (GnRH) (KGG) system in neuronal GT1-7 cells with mechanistic insights by Estrogen receptor signaling pathways. The study demonstrated that low-concentration Cl_xBPA induced the cell proliferation, promoted GnRH secretion, upregulated the expression of KGG neuroendocrine signal-related proteins (KissR1, GnRH1 and kisspeptin) and genes including Kiss1, GnRH1, KissR1, luteinizing hormone receptor (Lhr) and follicle-stimulating hormone receptor (Fshr) in GT1-7 cells. Additionally, Cl_xBPA activated nuclear Estrogen receptor alpha (ERα) and member Estrogen receptor G protein-coupled Estrogen receptor (GPER)-regulated phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt) and extracellular signal-regulated kinase (ERK1/2) signaling pathways. Pretreatment of GT1-7 cells with GPER inhibitor G15 and ERα Inhibitor ICI reduced the expression of KissR1, GnRH1 and kisspeptin proteins, attenuated mRNA levels of Kiss1, GnRH1, KissR1, Fshr and Lhr genes, and decreased Cl_xBPA-induced GT1-7 cell proliferation. The results suggested that Cl_xBPA activated the KGG neuroendocrine signals and induced the proliferation of GT1-7 cells via ERα and GPER signaling pathways. This study provides a new perspective to explore the neuroendocrine toxicity mechanism of Cl_xBPA. CAPSULE: Cl_xBPA activated KGG neuroendocrine signaling pathway via ERα and GPER and induced the proliferation of GT1-7 cells.

Chlorobisphenol A; G protein-coupled estrogen receptor; GT1–7 neuronal cells; Kisspeptin/GPR54-GnRH neuroendocrine system; Molecular mechanisms.