2. Academic Validation
  3. Identification of Antibacterial Metabolites from Endophytic Fungus Aspergillus fumigatus, Isolated from Albizia lucidior Leaves (Fabaceae), Utilizing Metabolomic and Molecular Docking Techniques

Identification of Antibacterial Metabolites from Endophytic Fungus Aspergillus fumigatus, Isolated from Albizia lucidior Leaves (Fabaceae), Utilizing Metabolomic and Molecular Docking Techniques

  • Molecules. 2022 Feb 8;27(3):1117. doi: 10.3390/molecules27031117.
Mai E Hussein 1,  Osama G Mohamed 1 2,  Ahlam M El-Fishawy 1,  Hesham I El-Askary 1,  Amira S El-Senousy 1,  Ahmed A El-Beih 3,  Eman S Nossier 4,  Ahmed M Naglah 5,  Abdulrahman A Almehizia 5,  Ashootosh Tripathi 2 6,  Ahmed A Hamed 7
Affiliations

Affiliations

  • 1 Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr el Aini St., Cairo 11562, Egypt.
  • 2 Natural Products Discovery Core, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA.
  • 3 Department of Chemistry of Natural and Microbial Products, National Research Centre, Dokki, Giza 12622, Egypt.
  • 4 Department of Pharmaceutical Medicinal Chemistry and Drug Design, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo 11754, Egypt.
  • 5 Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
  • 6 Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA.
  • 7 Microbial Chemistry Department, National Research Centre, 33 El-Buhouth Street, Dokki, Giza 12622, Egypt.
PMID: 35164382 DOI: 10.3390/molecules27031117
Abstract

The rapid spread of Bacterial infection caused by Staphylococcus aureus has become a problem to public health despite the presence of past trials devoted to controlling the Infection. Thus, the current study aimed to explore the chemical composition of the extract of endophytic fungus Aspergillus fumigatus, isolated from Albizia lucidior leaves, and investigate the antimicrobial activity of isolated metabolites and their probable mode of actions. The chemical investigation of the Fungal extract via UPLC/MS/MS led to the identification of at least forty-two metabolites, as well as the isolation and complete characterization of eight reported metabolites. The Antibacterial activities of isolated metabolites were assessed against S. aureus using agar disc diffusion and microplate dilution methods. Compounds ergosterol, helvolic acid and monomethyl sulochrin-4-sulphate showed minimal inhibitory concentration (MIC) values of 15.63, 1.95 and 3.90 µg/mL, respectively, compared to ciprofloxacin. We also report the inhibitory activity of the Fungal extract on DNA gyrase and Topoisomerase IV, which led us to perform molecular docking using the three most active compounds isolated from the extract against both enzymes. These active compounds had the required structural features for S. aureus DNA gyrase and Topoisomerase IV inhibition, evidenced via molecular docking.

Keywords

Albizia lucidior; Aspergillus fumigatus; DNA gyrase; UHPLC–QTOF; antibacterial; topoisomerase IV.

Figures
Products