2. Academic Validation
  3. Effect of ACY-1215 on cytoskeletal remodeling and histone acetylation in bovine somatic cell nuclear transfer embryos

Effect of ACY-1215 on cytoskeletal remodeling and histone acetylation in bovine somatic cell nuclear transfer embryos

  • Theriogenology. 2022 Apr 15;183:98-107. doi: 10.1016/j.theriogenology.2022.02.018.
Song Gao 1,  Zheng Wang 1,  Jukui Ma 1,  Jingyi Wang 2,  Xupeng Xing 3,  Chenglong Shen 1,  Zhihan Niu 1,  Heqiang Li 1,  Shaowei Zhang 1,  Kaijuan Zhang 1,  Jing Gao 1,  Yong Zhang 4,  Yongsheng Wang 5
Affiliations

Affiliations

  • 1 College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China, Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, Shaanxi, 712100, PR China.
  • 2 Department of Gynecology and Obstetrics, Tangdu Hospital, the Fourth Military Medical University, Xi'an, Shannxi, 710000, PR China.
  • 3 National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, 510000, PR China.
  • 4 College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China, Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, Shaanxi, 712100, PR China. Electronic address: [email protected].
  • 5 College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China, Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, Shaanxi, 712100, PR China. Electronic address: [email protected].
PMID: 35231828 DOI: 10.1016/j.theriogenology.2022.02.018
Abstract

The latest studies indicated that in addition to alterations in abnormal chromosome epigenetic modifications, the abnormal cytoskeletal changes are also an important cause for the developmental failure of somatic cell nuclear transfer (SCNT) embryos. In the present study, the effects of ACY-1215, a specific inhibitor of HDAC6, on the acetylation of α-tubulin, histone epigenetic modification, spindle formation and embryonic development of early bovine SCNT embryos were studied. The results showed that acetylation of α-tubulin, H3K9, and H4K16 was significantly lower in SCNT embryos than in vitro fertilization (IVF) embryos. After ACY-1215 treatment, the acetylation level of α-tubulin, H3K9, and H4K16 of SCNT embryos was closer to that of IVF embryos. ACY-1215 treatment reduced spindle abnormalities, delayed the time of first cleavage of embryos, increased the total cell number and trophectoderm cells numbers, and reduced Apoptosis in SCNT blastocysts. ACY-1215 regulated the process of embryonic epigenetic modification and cytoskeletal protein acetylation, corrected abnormal development of SCNT embryos, and improved SCNT embryonic development potential.

Keywords

Acetylated α-tubulin; Bovine; Histone acetylation modification; SCNT embryo.

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