2. Academic Validation
  3. PCR-based profiling of transcription factor activity in vivo by a virus-based reporter battery

PCR-based profiling of transcription factor activity in vivo by a virus-based reporter battery

  • iScience. 2022 Feb 14;25(3):103927. doi: 10.1016/j.isci.2022.103927.
Hitomi Abe 1,  Kentaro Abe 1 2
Affiliations

Affiliations

  • 1 Lab of Brain Development, Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai, Miyagi 980-8577, Japan.
  • 2 Division for the Establishment of Frontier Sciences, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai, Miyagi 980-8577, Japan.
PMID: 35281741 DOI: 10.1016/j.isci.2022.103927
Abstract

Understanding the molecular mechanisms of gene regulation is pivotal for understanding how cells establish and modify their identities and functions. Multiple transcription factors (TFs) coordinate to alter gene expression in cells; however, a method to quantitatively analyze the activity of each TF is lacking, particularly in vivo. Here, we introduce a viral-vector-based TF reporter battery that can be used to simultaneously analyze the activity of multiple TFs, visualized as the TF activity profile (TFAP) obtained by qPCR. We show that the cells possess distinct TFAPs that dynamically change according to experimental manipulation or physiological activity. We report a practical method to obtain the TFAP of a defined cell population and their experience-dependent changes in the mouse brain in vivo. The TFAP obtained by our method will help bridge the information gap between the genome and transcriptome and aid the multi-omics view of understanding the gene regulation system.

Keywords

Biological sciences; Biological sciences research methodologies; Biology experimental methods; Cell biology; Methodology in biological sciences; Neuroscience.

Figures
Products