2. Academic Validation
  3. Galactooligosaccharides as a protective agent for intestinal barrier and its regulatory functions for intestinal microbiota

Galactooligosaccharides as a protective agent for intestinal barrier and its regulatory functions for intestinal microbiota

  • Food Res Int. 2022 May:155:111003. doi: 10.1016/j.foodres.2022.111003.
Geng Wang 1,  Haidong Wang 2,  Yuyue Jin 3,  Zhiping Xiao 4,  Muhammad Umar Yaqoob 5,  Yueying Lin 6,  Haohan Chen 7,  Minqi Wang 8
Affiliations

Affiliations

  • 1 Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310058, PR China. Electronic address: [email protected].
  • 2 Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310058, PR China. Electronic address: [email protected].
  • 3 Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310058, PR China. Electronic address: [email protected].
  • 4 Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310058, PR China. Electronic address: [email protected].
  • 5 Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310058, PR China. Electronic address: [email protected].
  • 6 Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310058, PR China. Electronic address: [email protected].
  • 7 Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310058, PR China. Electronic address: [email protected].
  • 8 Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310058, PR China. Electronic address: [email protected].
PMID: 35400418 DOI: 10.1016/j.foodres.2022.111003
Abstract

Present study was conducted to comprehensively investigate the protective effects of galactooligosaccharides (GOS, 100%) against LPS-induced intestinal barrier damages, and the regulatory effect for intestinal microbes. Results showed that GOS intervention restored villi (jejunum and ileum) integrity, which were atrophic and broken in LPS-challenged mice. Electron microscopy, western blotting and immunofluorescence analysis exhibited that mice administrated with GOS showed higher expression of tight junction, which was confirmed in IPEC-J2 cells model. Meanwhile, the GOS increased the secretion of Mucin and SIgA, as well as it alleviated inflammatory response caused by LPS in NF-κB dependent way. Administration of GOS could also increase the relative abundances of several specific friendly bacteria, and enhance the adaptability of intestinal microbiota. Collectively, these results indicated the potential of GOS for protecting intestine from injuries caused by stress as LPS challenge.

Keywords

Galactooligosaccharides; Inflammatory responses; Intestinal microbes; Mucin; SIgA; Tight junction.

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