2. Academic Validation
  3. Gamma-oryzanol alleviates intervertebral disc degeneration development by intercepting the IL-1β/NLRP3 inflammasome positive cycle

Gamma-oryzanol alleviates intervertebral disc degeneration development by intercepting the IL-1β/NLRP3 inflammasome positive cycle

  • Phytomedicine. 2022 Jul 20:102:154176. doi: 10.1016/j.phymed.2022.154176.
Hui Xu 1,  Zi-Han Dai 1,  Gao-Lu He 1,  Han-Chen Cai 1,  Xuan-Yang Chen 1,  Yan-Lin Chen 2,  Cong Xu 3,  Sun-Ren Sheng 4
Affiliations

Affiliations

  • 1 Key Laboratory of Orthopaedics of Zhejiang Province, Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; The Second School of Medicine, Wenzhou Medical University, Wenzhou, Zhejian 325000, China.
  • 2 Department of Orthopaedics Surgery, Lishui Central Hospital and Fifth Affiliated Hospital of Wenzhou Medical University, Lishui, Zhejiang 323000, China. Electronic address: [email protected].
  • 3 Key Laboratory of Orthopaedics of Zhejiang Province, Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; The Second School of Medicine, Wenzhou Medical University, Wenzhou, Zhejian 325000, China. Electronic address: [email protected].
  • 4 Key Laboratory of Orthopaedics of Zhejiang Province, Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; The Second School of Medicine, Wenzhou Medical University, Wenzhou, Zhejian 325000, China. Electronic address: [email protected].
PMID: 35660354 DOI: 10.1016/j.phymed.2022.154176
Abstract

Background: Intervertebral disc degeneration (IVDD) is a highly prevalent musculoskeletal disorder characterized by a local inflammatory response associated with the IL-1β/NLRP3 inflammasome positive feedback loop. Rice bran-derived gamma-oryzanol (Ory) as a sterol ferulate has attracted much attention due to its powerful anti-inflammatory, hypoglycemic and hypolipidemic health effects. As a clinical pharmaceutical for autonomic disorders, Ory's role in musculoskeletal degenerative disease remains unknown.

Purpose: This study aims to validate the role of Ory in IVDD and explore the potential mechanism.

Study design: Establishing the in vitro and in vivo IVDD models to detect the protective effect and molecular mechanism of Ory.

Method: The anti-ECM degradation, antioxidant and anti-NLRP3 inflammasome activation effects of Ory on IL-1β-stimulated nucleus pulposus (NP) cells were assessed by immunoblotting and immunofluorescence, etc. MRI, S-O staining and immunohistochemistry were performed to estimate the effects of Ory administration on acupuncture-mediated IVDD in rats at imaging and histological levels.

Results: Ory treatment inhibited IL-1β-mediated ECM degradation, oxidative stress and NLRP3 inflammasome activation in NP cells. By interfering with NF-κB signaling and ROS overproduction, Ory interrupted IL-1β/NLRP3-inflammasome positive cycle. In vivo experiments showed that Ory delayed acupuncture-mediated IVDD development.

Conclusion: Our results support the potential application of Ory as a therapeutic compound for IVDD.

Keywords

Gamma-oryzanol; Intervertebral disc degeneration; NF-κB; NLRP3 inflammasome; Nucleus pulposus.

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