2. Academic Validation
  3. Synthetic Coelenterazine Derivatives and Their Application for Bioluminescence Imaging

Synthetic Coelenterazine Derivatives and Their Application for Bioluminescence Imaging

  • Methods Mol Biol. 2022:2524:17-36. doi: 10.1007/978-1-0716-2453-1_2.
Tianyu Jiang 1 2 3,  Minyong Li 4
Affiliations

Affiliations

  • 1 Department of Medicinal Chemistry, Key Laboratory of Chemical Biology of Natural Products (MOE), School of Pharmacy, Shandong University, Jinan, Shandong, China.
  • 2 State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, Shandong University, Qingdao, Shandong, China.
  • 3 Shenzhen Research Institute of Shandong University, Shenzhen, Guangdong, China.
  • 4 Department of Medicinal Chemistry, Key Laboratory of Chemical Biology of Natural Products (MOE), School of Pharmacy, Shandong University, Jinan, Shandong, China. [email protected].
PMID: 35821460 DOI: 10.1007/978-1-0716-2453-1_2
Abstract

Bioluminescence (BL), the emission light resulting from the enzyme-catalyzed oxidative reaction, is a powerful imaging modality for monitoring biological phenomena both in vitro and in vivo. Coelenterazine (CTZ), the known widespread luciferin found in bioluminescent organisms, develops bioluminescence imaging (BLI). Here, we describe an approach to synthesize a series of novel CTZ derivatives for diversifying the toolbox of the BL substrates. Furthermore, we exemplify some of them display excellent BL signals in vitro and in vivo, and thus should be noted as one of the ideal substrates for in vivo BLI compared with a well-known conventional substrate, DeepBlueC.

Keywords

Bioluminescence; Coelenterazine (CTZ); Derivatives; Luciferin; Renilla luciferase (RLuc).

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