2. Academic Validation
  3. OL-FS13 alleviates experimental cerebral ischemia-reperfusion injury

OL-FS13 alleviates experimental cerebral ischemia-reperfusion injury

  • Exp Neurol. 2022 Nov;357:114180. doi: 10.1016/j.expneurol.2022.114180.
Naixin Liu 1,  Yilin Li 1,  Ying Yang 2,  Longjun Shu 3,  Yixiang Liu 3,  Yutong Wu 1,  Dandan Sun 1,  Zijian Kang 1,  Yue Zhang 1,  Dan Ni 1,  Ziqi Wei 1,  Shanshan Li 1,  Meifeng Yang 1,  Ying Wang 4,  Jun Sun 5,  Xinwang Yang 6
Affiliations

Affiliations

  • 1 Department of Anatomy and Histology & Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming 650500, Yunnan, China.
  • 2 Endocrinology Department of affiliated Hospital of Yunnan University, Kunming 650021, Yunnan, China.
  • 3 Key Laboratory of Chemistry in Ethnic Medicinal Resources & Key Laboratory of Natural Products Synthetic Biology of Ethnic Medicinal Endophytes, State Ethnic Affairs Commission & Ministry of Education, School of Ethnic Medicine, Yunnan Minzu University, Kunming 650504, Yunnan, China.
  • 4 Key Laboratory of Chemistry in Ethnic Medicinal Resources & Key Laboratory of Natural Products Synthetic Biology of Ethnic Medicinal Endophytes, State Ethnic Affairs Commission & Ministry of Education, School of Ethnic Medicine, Yunnan Minzu University, Kunming 650504, Yunnan, China. Electronic address: [email protected].
  • 5 Department of Anatomy and Histology & Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming 650500, Yunnan, China. Electronic address: [email protected].
  • 6 Department of Anatomy and Histology & Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming 650500, Yunnan, China. Electronic address: [email protected].
PMID: 35901974 DOI: 10.1016/j.expneurol.2022.114180
Abstract

Cerebral ischemia-reperfusion (I/R) is the main cause of neurological injury after stroke. However, existing treatments for I/R injury are relatively poor, and relevant drugs need to be further explored. Amphibians have received increasing attention as a resource bank of bioactive Peptides. However, reports on neuroprotective Peptides from amphibians remain extremely rare. Here, we identified a new neuroprotective peptide (OL-FS13, amino acid sequence: FSLLLTWWRRRVC) from the odorous frog species Odorrana livida using a constructed cDNA library. OL-FS13 significantly improving infarct volume, behavioral and histological abnormalities in rats, and also showed neuroprotective activities in PC12 cell (by oxygen glucose deprivation/reoxygenation, OGD/R). Mechanistically, OL-FS13 increased the level of antioxidative enzymes to resist oxidative stress and alleviated endoplasmic reticulum (ER) stress induced by I/R and OGD/R. The use of ML385 (Nrf2 inhibitor) indicated that OL-FS13 relieved nerve damage caused by oxidative and ER stress by increasing the nuclear displacement of Nrf2. Collectively, this research provides a novel drug candidate for the clinical cerebral I/R curation.

Keywords

Bioactive peptide; Cerebral ischemia; Neuroprotection; Oxidative stress; Reperfusion.

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