1. NF-κB
    Apoptosis
  2. Keap1-Nrf2
    Ferroptosis
  3. ML385

ML385 

Cat. No.: HY-100523 Purity: 99.55%
Handling Instructions

ML385 is a specific nuclear factor erythroid 2-related factor 2 (NRF2) inhibitor with an IC50 of 1.9 μM.

For research use only. We do not sell to patients.

ML385 Chemical Structure

ML385 Chemical Structure

CAS No. : 846557-71-9

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Solution
10 mM * 1 mL in DMSO USD 68 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 68 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 60 In-stock
Estimated Time of Arrival: December 31
10 mg USD 90 In-stock
Estimated Time of Arrival: December 31
25 mg USD 190 In-stock
Estimated Time of Arrival: December 31
50 mg USD 310 In-stock
Estimated Time of Arrival: December 31
100 mg USD 490 In-stock
Estimated Time of Arrival: December 31
200 mg USD 750 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 105 publication(s) in Google Scholar

Top Publications Citing Use of Products

Publications Citing Use of MCE ML385

    ML385 purchased from MCE. Usage Cited in: Exp Gerontol. 2017 Dec 15;100:77-86.

    Cells are treated with ML385 at the concentration of 5 μM for 12 h. The expression of Nrf2 and its translocation into nucleus are determined using western blot analysis.

    ML385 purchased from MCE. Usage Cited in: Biochim Biophys Acta. 2017 Dec 26;1864(3):882-890.

    RAW264.7 cells are transfected with ML385 (5 μM) in the presence of Tan C (10 μM) for 24 h. Prdx1 and Nrf2 protein expression is determined by Western blotting

    ML385 purchased from MCE. Usage Cited in: Br J Pharmacol. 2018 Oct;175(19):3747-3757.

    Western blot of whole liver homogenate for p-JNK and 3-NT in mice with or without Chloroquine (CQ; 60 mg/kg) at 6 h.

    ML385 purchased from MCE. Usage Cited in: Biochem Pharmacol. 2018 Sep;155:494-509.

    THP-1 cells are incubated with Cardamonin (30 μM), ML385 (5 μM) and ML385 + Cardamonin for 21 h, stimulated with LPS (2 μg/mL) for 3 h, and followed by ATP (5 mM) treatment for 30 min. The protein levels of NLRP3, cleaved caspase-1, ASC and cleaved IL-1β are detected by western blotting assay.

    ML385 purchased from MCE. Usage Cited in: Aging (Albany NY). 2018 Aug 16;10(8):2016-2036.

    Relative changes in the expression of Nrf2, pNrf2 and HO-1 after treatment with D-gal, ML385 alone or combined with lycopene.

    ML385 purchased from MCE. Usage Cited in: Nat Commun. 2018 Oct 24;9(1):4429. 

    QPCR detecting NQO1 and GCLC genes in vector or HPV16 E7-expressing HaCaT cells treated with or without 10 μM ML385 for 24 h.

    ML385 purchased from MCE. Usage Cited in: Front Pharmacol. 2018 Dec 13;9:1444.

    Seven days after the last MPTP intoxication, nucleus, and cytosol isolated from SNpc and striatum from all groups (sham, KOK, KOK+ML385, ML385, KOK, ML385) are subjected to Western blot assay to quantify changes in Nrf2 nuclear translocation and NQO1 expression, respectively.

    ML385 purchased from MCE. Usage Cited in: Front Pharmacol. 2018 Dec 13;9:1444.

    Seven days after the last MPTP intoxication, SNpc and striatal sections (n = 3 per brain) are subjected to immunofluorescence staining using Nrf2 and NQO1 antibodies with the treatments (sham, KOK, KOK+ML385, ML385, KOK, ML385).

    ML385 purchased from MCE. Usage Cited in: Oxid Med Cell Longev. 2019 Mar 18;2019:2853534.

    The protein expression levels of Nrf2 (nucleus), Nrf2 (cytoplasm), HO-1, p-NF-κB p65, NF-κB p65, and ICAM-1 proteins are analyzed by Western blot with or without the treatment of H2O2, SAA and ML385.

    ML385 purchased from MCE. Usage Cited in: Oxid Med Cell Longev. 2019 Apr 7;2019:3501059.

    Western blot of Nrf2 and HO-1 protein expression levels with the treatment of HEMA, HEMA+BHQ, and HEMA+ML385.

    ML385 purchased from MCE. Usage Cited in: Oxid Med Cell Longev. 2019 Apr 7;2019:3501059.

    Immunofluorescence analysis of Nrf2 expression and nuclear translocation. Green fluorescence represents Nrf2, and blue fluorescence represents nucleus of hDPCs. Data represent mean ± standard deviations (n = 3).
    • Biological Activity

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    Description

    ML385 is a specific nuclear factor erythroid 2-related factor 2 (NRF2) inhibitor with an IC50 of 1.9 μM.

    IC50 & Target

    IC50: 1.9 μM (NRF2)[1]

    In Vitro

    ML385 interacts with NRF2 and affects the DNA binding activity of the NRF2-MAFG protein complex. The addition of ML385 decreases anisotropy in a dose-dependent manner, with an IC50 of 1.9 μM. A dose-dependent reduction in the NRF2 transcriptional activity is observed and the maximum inhibitory concentration is 5 μM by ML385. Treatment with ML385 leads to a significant reduction in NRF2 and downstream target gene expression selectively in KEAP1 mutant H460 cells. ML385 selectively affects the colony forming ability or growth of lung cancer cells with gain of NRF2 function[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    ML385 in combination with carboplatin leads to a significant reduction in tumor cell proliferation, demonstrated by fewer Ki-67 positive cells. Tumor samples treated with ML385 show a significant reduction in NRF2 protein level and its downstream target genes[1].
    ML385 (intraperitoneal injection; 30 mg/kg; 7 days) weakens the therapeutic effects of MSC-Exo on inflammation-induced astrocytic activation in mice, and reduces reactive astrogliosis, NF-κB deactivation[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 8-week-old C57B/6 male mice[3]
    Dosage: 30 mg/kg; 7 days
    Administration: Intraperitoneal injection
    Result: Reversed inhibition of MSC-Exo on hippocampal astrocytic activation in vivo.
    Molecular Weight

    511.59

    Formula

    C₂₉H₂₅N₃O₄S

    CAS No.
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (97.73 mM; Need ultrasonic)

    H2O : 0.1 mg/mL (0.20 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9547 mL 9.7735 mL 19.5469 mL
    5 mM 0.3909 mL 1.9547 mL 3.9094 mL
    10 mM 0.1955 mL 0.9773 mL 1.9547 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 6.97 mg/mL (13.62 mM); Clear solution

    • 2.

      Add each solvent one by one:  15% Solutol HS 15  10% Cremophor EL  35% PEG 400   40% water

      Solubility: 5 mg/mL (9.77 mM); Suspended solution; Need ultrasonic

    • 3.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: 3.25 mg/mL (6.35 mM); Suspended solution; Need ultrasonic

    *All of the co-solvents are provided by MCE.
    References

    Purity: 99.55%

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    Product Name:
    ML385
    Cat. No.:
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