1. GPCR/G Protein
    Neuronal Signaling
    Autophagy
    Metabolic Enzyme/Protease
  2. Melatonin Receptor
    Autophagy
    Mitophagy
    Endogenous Metabolite
  3. Melatonin

Melatonin (Synonyms: N-Acetyl-5-methoxytryptamine)

Cat. No.: HY-B0075 Purity: 98.95%
Handling Instructions

Melatonin is a hormone made by the pineal gland that can activates melatonin receptor. Melatonin plays a role in sleep and possesses important antioxidative and anti-inflammatory properties.

For research use only. We do not sell to patients.

Melatonin Chemical Structure

Melatonin Chemical Structure

CAS No. : 73-31-4

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
1 g USD 60 In-stock
Estimated Time of Arrival: December 31
5 g USD 126 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 6 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Melatonin purchased from MCE. Usage Cited in: Molecules. 2018 Mar 16;23(3). pii: E675.

    Representative image of immunobloting of UCP2 in the cultured AC16 cells under various treatments.

    Melatonin purchased from MCE. Usage Cited in: J Cell Mol Med. 2018 Oct;22(10):5132-5144.

    Melatonin regulation of mitophagy activity in the diabetic myocardium, Protein expression with representative gel blots of LC3 I/II, GAPDH (loading control).
    • Biological Activity

    • Protocol

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    • References

    • Customer Review

    Description

    Melatonin is a hormone made by the pineal gland that can activates melatonin receptor. Melatonin plays a role in sleep and possesses important antioxidative and anti-inflammatory properties.

    In Vivo

    Melatonin increases the levels of activated PTEN, RSK-1, mTOR and AMPKα kinases, mildly inhibits ERK-1/2 phosphorylation and Bad phosphorylation, significantly inhibits phosphorylations of S6 Ribosomal Protein, 4E-BP1, GSK-3α and GSK-3β, and slightly increases PRAS40 phosphorylation in animals[1]. Melatonin ameliorates the neurotoxiciy and astrocyte activation induced by Aβ1-42 in the cerebral cortex. Melatonin also blocks the reduction in Reelin and Dab1 expression induced by Aβ1-42[2]. Melatonin treatment and lack of NLRP3-/- share similar inhibition of NF-κB and NLRP3 signaling pathway in mice. Melatonin treatment and lack of NLRP3-/- share some patterns of clock genes expression, and improve cardiomyocytes morphology in mice[3].

    Clinical Trial
    Molecular Weight

    232.28

    Formula

    C₁₃H₁₆N₂O₂

    CAS No.

    73-31-4

    SMILES

    CC(NCCC1=CNC2=C1C=C(OC)C=C2)=O

    Shipping

    Room temperature in continental US; may vary elsewhere

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 68 mg/mL (292.75 mM)

    Ethanol : ≥ 50 mg/mL (215.26 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.3051 mL 21.5257 mL 43.0515 mL
    5 mM 0.8610 mL 4.3051 mL 8.6103 mL
    10 mM 0.4305 mL 2.1526 mL 4.3051 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (10.76 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% EtOH    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (10.76 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% EtOH    90% corn oil

      Solubility: ≥ 2.5 mg/mL (10.76 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Animal Administration
    [1]

    A total of two sets of adult male C57BL/6j mice weighing 21-26 g are randomly assigned to one of four groups and treated with intraperitoneal (i.p.) delivery of (i) vehicle (50 μL isotonic saline/5% ethanol), (ii) melatonin (4 mg/kg, dissolved in 0.9% isotonic saline/5% ethanol), (iii) Wortmannin, and (iv) melatonin/Wortmannin immediately after reperfusion. In the first set, mice are exposed to 30 min of focal cerebral ischemia (FCI) and 72 h reperfusion for the evaluation of disseminate ischemic injury in the striatum, and signaling pathway analysis (n=7 per group). The second group of mice is exposed to 90 min of FCI and 24 h reperfusion for the analysis of infarct development, brain swelling and IgG extravasation (n=7 per group).

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 98.95%

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    Product Name:
    Melatonin
    Cat. No.:
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