L-Tryptophan (Synonyms: Tryptophan; Tryptophane)

Name: L-Tryptophan
Brand: MedChemExpress
SKU: HY-N0623
Rating: 4.5 (4 reviews)

Cat. No.: HY-N0623 Purity: 99.87%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

L-Tryptophan (Tryptophan) is an orally active and essential amino acid that is the precursor of serotonin, melatonin, and vitamin B3. L-Tryptophan can promote an increase in stemness and osteogenic ability of BMSCs in vitro and in vivo. L-Tryptophan inhibits cell proliferation and induced cell cycle arrest with high levels.

For research use only. We do not sell to patients.

L-Tryptophan Chemical Structure

CAS No. : 73-22-3

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of L-Tryptophan:

3 Publications Citing Use of MCE L-Tryptophan

: L-Tryptophan purchased from MedChemExpress. Usage Cited in: Obesity. 2023 Mar 30. [Abstract]; L-Trp (0.7 mM; 48 h) enhances the PA (palmitic acid) increased expression of PDX-1, NKX6.1, and MAFA in MIN6 cells. whereas CLP-3094 (CLP; 0.7 μM; 48 h) causes an opposing effect.

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Description

In Vitro

L-Tryptophan (Tryptophan; 500, 1000 μM; 48 h) with high levels markedly suppresses pTr2 cell proliferation and increases the percentages of cells in the G1 phase of cell cycle^[1].
L-Tryptophan (10 μM; for three weeks) enhances migration, colony formation and osteogenic differentiation of hBMSCs in vitro^[2].
L-Tryptophan (10 μM; for three weeks) decreases mRNA levels of adipogenic markers PPARγ and LPL^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	pTr2 cell
Concentration:	500, 1000 μM
Incubation Time:	48 h
Result:	500 and 1000 μM groups had decreased cell viability by 7% and 22%, respectively, at 48 h posttreatment.

Cell Cycle Analysis^[1]

Cell Line:	pTr2 cell
Concentration:	500, 1000 μM
Incubation Time:	48 h
Result:	The percentage of pTr2 cells in the G1 phase increased from 65.70% (control) to 71.37% (500 mM) and 73.86% (1000 mM).

Cell Migration Assay ^[2]

Cell Line:	hBMSCs
Concentration:	10 μM
Incubation Time:	For three weeks
Result:	Exhibited a higher ability for migration than the control group.

RT-PCR^[2]

Cell Line:	hBMSCs
Concentration:	10 μM
Incubation Time:	For three weeks
Result:	Decreased mRNA levels of adipogenic markers PPARγ and LPL.

In Vivo

L-Tryptophan (Tryptophan; 10, 50 kg/mg/day; Intraperitoneally; for consecutive three weeks) enhances migration, colony formation and osteogenic differentiation of mBMSCs in vivo with 50 mg/kg/day^[1].
L-Tryptophan (50 mg/kg/day; Intraperitoneally; for consecutive three weeks) accelerates bone regeneration in mouse femur surgical defect^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Five-week-old C57BL/6 mice^[2]
Dosage:	10, 50 kg/mg
Administration:	Intraperitoneally; daily; for consecutive three weeks
Result:	The concentration of 50 mg/kg/day induced a significant increase in the number of SSEA-4 positive cells. Increased the mRNA levels of Nanog and Oct-4 and the colony-forming ability of mBMSCs with 50 mg/kg/day.

Clinical Trial

Molecular Weight

204.23

Formula

C₁₁H₁₂N₂O₂

CAS No.

73-22-3

Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](CC1=CNC2=CC=CC=C12)C(O)=O

Structure Classification

Amino acids

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 7.69 mg/mL (37.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 5 mg/mL (24.48 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.8964 mL	24.4822 mL	48.9644 mL
5 mM	0.9793 mL	4.8964 mL	9.7929 mL
10 mM	0.4896 mL	2.4482 mL	4.8964 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.77 mg/mL (3.77 mM); Clear solution

This protocol yields a clear solution of ≥ 0.77 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (7.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.77 mg/mL (3.77 mM); Clear solution

This protocol yields a clear solution of ≥ 0.77 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (7.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.77 mg/mL (3.77 mM); Clear solution

This protocol yields a clear solution of ≥ 0.77 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (7.7 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 6.25 mg/mL (30.60 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Protocol 2

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 20 mg/mL (97.93 mM); Clear solution; Need ultrasonic and warming and heat to 45°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.87%

Select Batch:

Data Sheet (282 KB) SDS (0 KB)

COA (254 KB) HNMR (130 KB) RP-HPLC (226 KB)

Handling Instructions (2659 KB)

References

[1]. Kang Xu, et al. L-Tryptophan activates the aryl hydrocarbon receptor and induces cell cycle arrest in porcine trophectoderm cells. Theriogenology. 2021 Sep 1:171:137-146. [Content Brief]

[2]. Hai Thanh Pham, et al. Tryptophan and Kynurenine Enhances the Stemness and Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stromal Cells In Vitro and In Vivo. Materials (Basel). 2021 Jan 4;14(1):208. [Content Brief]

[3]. Slominski A, et al. Conversion of L-tryptophan to serotonin and melatonin in human melanoma cells. FEBS Lett. 2002 Jan 30;511(1-3):102-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	4.8964 mL	24.4822 mL	48.9644 mL	122.4110 mL
	5 mM	0.9793 mL	4.8964 mL	9.7929 mL	24.4822 mL
	10 mM	0.4896 mL	2.4482 mL	4.8964 mL	12.2411 mL
	15 mM	0.3264 mL	1.6321 mL	3.2643 mL	8.1607 mL
	20 mM	0.2448 mL	1.2241 mL	2.4482 mL	6.1206 mL
DMSO	25 mM	0.1959 mL	0.9793 mL	1.9586 mL	4.8964 mL
DMSO	30 mM	0.1632 mL	0.8161 mL	1.6321 mL	4.0804 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-Tryptophan73-22-3Tryptophan TryptophaneOthersOrallyessential amino acidserotoninmelatoninvitamin B3stemnessosteogenicBMSCsproliferationcell cycle arrestInhibitorinhibitorinhibit

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L-Tryptophan (Synonyms: Tryptophan; Tryptophane)

Customer Review

Other Forms of L-Tryptophan:

L-Tryptophan Related Antibodies

3 Publications Citing Use of MCE L-Tryptophan

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

L-Tryptophan Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

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