2. Academic Validation
  3. ALDH6A1 weakens the progression of colon cancer via modulating the RAS/RAF/MEK/ERK pathway in cancer cell lines

ALDH6A1 weakens the progression of colon cancer via modulating the RAS/RAF/MEK/ERK pathway in cancer cell lines

  • Gene. 2022 Oct 30;842:146757. doi: 10.1016/j.gene.2022.146757.
Xiang Li 1,  Nan Wang 2,  Yutong Wu 3,  Yidan Liu 4,  Ruoyu Wang 5
Affiliations

Affiliations

  • 1 The Second Department of Oncology, Affiliated Zhongshan Hospital of Dalian University, China.
  • 2 The Tenth Department of Proctology Department, Dalian University Affiliated Xinhua Hospital, China.
  • 3 Graduate School, Dalian Medical University, China.
  • 4 Stomatology Department, Affiliated Zhongshan Hospital of Dalian University, China.
  • 5 Oncology Department, Affiliated Zhongshan Hospital of Dalian University, China. Electronic address: [email protected].
PMID: 35907565 DOI: 10.1016/j.gene.2022.146757
Abstract

Background: Aldehyde dehydrogenase 6 family member A1 (ALDH6A1) is associated with multiple diseases, but its pathogenesis in colon Cancer (CC) is ambiguous and needs further study so that this research explores the function of ALDH6A1 in CC.

Methods: The level of ALDH6A1 in colon adenocarcinoma (COAD), CC tissues, and cells was measured by starBase v2.0, quantitative real-time polymerase chain reaction (qRT-PCR), and western blot. Post transfection with overexpressed (oe)-ALDH6A1, cell biological behaviors, as well as apoptosis-, matrix metalloproteinase (MMP)-, and rat sarcoma virus (Ras)/rapidly accelerated fibrosarcoma (Raf)/mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) pathway-related markers were measured by cell function experiments, qRT-PCR, and western blot. Next, the effects of small interfering RNA targeting ALDH6A1 (si-ALDH6A1) and Ras/Raf Inhibitor (MCP110) on cell biological behaviors, as well as apoptosis-, MMP-, and Ras/Raf/MEK/ERK pathway-related markers were detected again.

Results: ALDH6A1 was low-expressed in COAD, CC tissues, and cells. Oe-ALDH6A1 weakened cell vitality, migration and invasionbut facilitated apoptosis; while it reduced expression levels of Bcl-2, MMP-2, MMP-9 and the Ras/Raf/MEK/ERK pathway-related markers but promoted Bax level. However, the regulation of si-ALDH6A1 on cell biological behaviors and related genes was opposite to that of oe-ALDH6A1. Moreover, MCP110 rescued the regulation of si-ALDH6A1 on cell biological behaviors, expressions of apoptosis- MMP- as well as Ras/Raf/MEK/ERK pathway-related markers. To sum up, ALDH6A1 attenuated CC progression by down-regulating the expressions of Ras/Raf/MEK/ERK pathway-related markers.

Keywords

Aldehyde dehydrogenase 6 family member A1; Colon cancer; Rapidly accelerated fibrosarcoma; Rat sarcoma virus.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-123673
    99.82%, RAS-RAF Inhibitor
    Ras; Raf