1. Academic Validation
  2. Tetrazolium-Based Visually Indicating Bacteria Sensor for Colorimetric Detection of Point of Contamination

Tetrazolium-Based Visually Indicating Bacteria Sensor for Colorimetric Detection of Point of Contamination

  • ACS Appl Mater Interfaces. 2022 Aug 24;14(33):38153-38161. doi: 10.1021/acsami.2c08613.
Eugene Song 1 Kyeongeun Lee 1 2 Jooyoun Kim 1 3
Affiliations

Affiliations

  • 1 Department of Textiles, Merchandising and Fashion Design, Seoul National University, Seoul 08826, Korea.
  • 2 Reliability Assessment Center, FITI Testing & Research Institute, Seoul 07791, Korea.
  • 3 Research Institute of Human Ecology, Seoul National University, Seoul 08826, Korea.
Abstract

Protective equipment for detecting Bacterial contamination has been in high demand with increasing interest in public health and hygiene. Herein, a fiber-based visually indicating bacteria sensor (VIBS) embedded with iodonitrotetrazolium chloride is developed for the general purpose of detecting live bacteria, and its chromogenic effectiveness is investigated for Gram-negative Escherichia coli and Gram-positive Micrococcus luteus. The developed color intensity is measured by the LIGHT absorption coefficient to the scattering coefficient (K/S) based on the Kubelka-Munk equation, and the colorimetric sensitivities of different membranes are examined by calculating the limit of detection (LOD) and the limit of quantification (LOQ). The results demonstrate that the interactions between VIBS and bacteria depend on the wetting properties of membranes. A hydrophobic membrane shows excessive interactions at high concentrations of Gram-negative E. coli bacteria, whose cell membrane is lipophilic. The membrane blended with hydrophobic and hydrophilic Polymers displays linear colorimetric responses for both Gram-negative and Gram-positive bacteria strains, demonstrating a reliable sensing capability in the range of the tested bacteria concentration. This study is significant in that explorative experimentations are performed to conceive a proof of concept of a fiber-based bacteria sensor, which is readily applicable in various fields where bacteria pose a threat.

Keywords

bacteria; fiber membrane; point of contamination; sensitivity; visual sensor.

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