2. Academic Validation
  3. MRI measurement of alanine uptake in a mouse xenograft model of U-87 MG glioblastoma

MRI measurement of alanine uptake in a mouse xenograft model of U-87 MG glioblastoma

  • Magn Reson Imaging. 2022 Nov;93:189-194. doi: 10.1016/j.mri.2022.08.015.
Seung-Hyun Yang 1,  Yuna Choi 1,  Mirae Park 1,  Hye-Young Son 2,  Yong-Min Huh 3,  Chan Gyu Joo 4
Affiliations

Affiliations

  • 1 Department of Radiology, College of Medicine, Yonsei University, Seoul 03722, Republic of Korea.
  • 2 Department of Radiology, College of Medicine, Yonsei University, Seoul 03722, Republic of Korea; YUHS-KRIBB Medical Convergence Research Institute, Seoul 03722, Republic of Korea.
  • 3 Department of Radiology, College of Medicine, Yonsei University, Seoul 03722, Republic of Korea; YUHS-KRIBB Medical Convergence Research Institute, Seoul 03722, Republic of Korea; Department of Biochemistry & Molecular Biology, College of Medicine, Yonsei University, Seoul 03722, Republic of Korea. Electronic address: [email protected].
  • 4 Severance Biomedical Science Institute, College of Medicine, Yonsei University, Seoul 03722, Republic of Korea. Electronic address: [email protected].
PMID: 36029935 DOI: 10.1016/j.mri.2022.08.015
Abstract

The potential use of alanine as an MRI contrast agent was investigated. The relaxation properties of alanine solutions were measured at 9.4 T. The T2 relaxivity caused by the chemical exchange (R2ex) between amine protons and water protons was 0.10 mM-1 s-1 at 37 °C. As a demonstration, alanine uptake in a mouse xenograft model of U-87 MG glioblastoma was measured using MRI, and was compared with immunohistochemistry staining of ASCT2, a transporter that imports Amino acids into Cancer cells. Statistically significant (p = 0.0079) differences in ASCT2 distribution were found between regions that show strong and weak alanine uptake in MRI. To better understand the influence of perfusion, the effect of ASCT2 inhibition on the alanine uptake in MRI was investigated, and dynamic contrast enhanced MRI was compared with alanine MRI.

Keywords

ASCT2; Alanine; Amino acid contrast agent; Amino acid transporter; MRI; cancer metabolism.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-112683
    99.88%, ASCT2 Antagonist