2. Academic Validation
  3. Measuring Urinary 6-Sulphatoxymelatonin in Humans

Measuring Urinary 6-Sulphatoxymelatonin in Humans

  • Methods Mol Biol. 2022:2550:21-28. doi: 10.1007/978-1-0716-2593-4_4.
Melissa A St Hilaire 1 2,  Steven W Lockley 3 4 5
Affiliations

Affiliations

  • 1 Division of Sleep and Circadian Disorders, Department of Medicine, Brigham and Women's Hospital, Boston, MA, USA.
  • 2 Division of Sleep Medicine, Harvard Medical School, Boston, MA, USA.
  • 3 Division of Sleep and Circadian Disorders, Department of Medicine, Brigham and Women's Hospital, Boston, MA, USA. [email protected].
  • 4 Division of Sleep Medicine, Harvard Medical School, Boston, MA, USA. [email protected].
  • 5 Surrey Sleep Research Centre, Department of Clinical and Experimental Medicine, Faculty of Health and Medical Sciences, University of Surrey, Guildford, UK. [email protected].
PMID: 36180673 DOI: 10.1007/978-1-0716-2593-4_4
Abstract

The pineal melatonin rhythm provides a robust reference signal for the timing of the endogenous human circadian system. The rhythm in the major urinary metabolite of melatonin, 6-sulphatoxymelatonin (aMT6s), is highly correlated with plasma melatonin and provides a noninvasive method to measure circadian phase, particularly in field-based studies. In this chapter, we describe the protocol for collecting urinary aMT6s and the method used to calculate the acrophase, or peak, time as a circadian phase marker.

Keywords

6-sulphatoxymelatonin; Circadian; Human; Melatonin; Urine.

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