2. Academic Validation
  3. MiR-9a-5p alleviates ventilator-induced lung injury in rats by inhibiting the activation of the MAPK signaling pathway via CXCR4 expression downregulation

MiR-9a-5p alleviates ventilator-induced lung injury in rats by inhibiting the activation of the MAPK signaling pathway via CXCR4 expression downregulation

  • Int Immunopharmacol. 2022 Nov;112:109288. doi: 10.1016/j.intimp.2022.109288.
Shuang He 1,  Zongyu Chen 2,  Chunju Xue 2,  Leilei Zhou 2,  Chunyu Li 2,  Wenqing Jiang 2,  Siyu Lian 2,  Yi Shen 2,  Minghua Liao 2,  Xianming Zhang 3
Affiliations

Affiliations

  • 1 School of Clinical Medicine, Guizhou Medical University, 550004 Guiyang, China. Electronic address: [email protected].
  • 2 School of Clinical Medicine, Guizhou Medical University, 550004 Guiyang, China.
  • 3 Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Guizhou Medical University, 550004 Guiyang, China. Electronic address: [email protected].
PMID: 36193608 DOI: 10.1016/j.intimp.2022.109288
Abstract

Background: Globally, Mechanical ventilation is the most commonly used short-term life support technology. Ventilator-induced lung injury (VILI) is an inflammatory injury caused by mechanical ventilation. MicroRNAs (miRNAs) are considered as new gene regulators that play an important role in lung injury and inflammation. However, the role and mechanism of action of miR-9a-5p in VILI remain unclear.

Methods: Herein, a rat model of VILI was established. To determine the expression levels of miR-9a-5p and CXCR4 mRNA, real-time quantitative polymerase chain reactions (qRT-PCR) were conducted. As well as western blot (WB) and immunofluorescence analyses, we determined the expression of CXCR4, SDF-1 and MAPK signaling pathway-related kinases. Hematoxylin and eosin (H&E) staining and the wet-dry ratio of the lung tissue were used to evaluate organ injury. An enzyme-linked immunosorbent assay (Elisa) and myeloperoxidase (MPO) activity measurements were performed to evaluate the inflammatory response. In addition, double luciferase reporter assays were used to verify the association between miR-9a-5p and CXCR4.

Results: The expression of miR-9a-5p was low, whereas that of CXCR4 was high in the lung tissues of VILI rats. The overexpression of miR-9a-5p alleviated the degree of pathological injury in the lung tissues of rats with VILI, downregulating inflammatory cytokine expression and MPO activity. In the VILI rat model, miR-9a-5p targeted the negative regulation of CXCR4, and CXCR4 overexpression to reverse the lung-protective and anti-inflammatory effects of miR-9a-5p overexpression in VILI rats. miR-9a-5p also inhibited the phosphorylation of extracellular signal receptor-activated kinase (ERK), a protein related to the MAPK signaling pathway, by downregulating CXCR4 expression.

Conclusion: miR-9a-5p can hinder the activation of the MAPK/ERK signaling pathway and reduce inflammatory reactions and lung injury in VILI rats through the targeted regulation of CXCR4 expression. Therefore, miR-9a-5p could serve as an intervention target to supply a new strategy for the care of VILI.

Keywords

CXCR4; Inflammation; Ventilator-induced lung injury; miR-9a-5p.

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