2. Academic Validation
  3. The contribution of spinal dorsal horn astrocytes in neuropathic pain at the early stage of EAE

The contribution of spinal dorsal horn astrocytes in neuropathic pain at the early stage of EAE

  • Neurobiol Dis. 2022 Nov 1;175:105914. doi: 10.1016/j.nbd.2022.105914.
Yumeng Ding 1,  Liye Hu 2,  Xiaotong Wang 1,  Qingyu Sun 1,  Tingting Hu 1,  Jing Liu 1,  Danmin Shen 2,  Yurui Zhang 1,  Wen Chen 1,  Chao Wei 1,  Meng Liu 1,  Dianxin Liu 1,  Peipei Wang 1,  Chenguang Zhang 2,  Jiannan Zhang 1,  Qian Li 3,  Fei Yang 4
Affiliations

Affiliations

  • 1 Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.
  • 2 Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.
  • 3 Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China; Advanced Innovation Center for Human Brain Protection, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing 100069, China. Electronic address: [email protected].
  • 4 Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China; Advanced Innovation Center for Human Brain Protection, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing 100069, China. Electronic address: [email protected].
PMID: 36332813 DOI: 10.1016/j.nbd.2022.105914
Abstract

Reactive astrocytes play a complex role in multiple sclerosis, and the astrocytes reactivity is an important factor in the pathogenesis of pain. It is of great significance to explore the genesis and development mechanism of pain in the early stage of multiple sclerosis (MS) for early intervention of the disease. This study aims to explore astrocyte reactivity at different stages of the experimental autoimmune encephalomyelitis (EAE) model, a mouse model of MS, and the role of astrocytes in the pain in the early stage of the EAE. In this study, we demonstrated that spinal dorsal horn astrocytes were activated in the pre-clinical stage of EAE mice, and the inhibition of spinal cord astrocyte reactivity effectively alleviates pain symptoms in EAE mice. On the other hand, spinal cord microglia were not directly participated in the early EAE pain. Moreover, the ion channel LRRC8A mediated the reactivity of spinal dorsal horn astrocytes by regulating the STAT3 pathway, therefore playing a role in the early pain of EAE.

Keywords

Astrocyte; Experimental autoimmune encephalomyelitis; LRRC8A; Multiple sclerosis; Pain.

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