2. Academic Validation
  3. DLP-based bioprinting of void-forming hydrogels for enhanced stem-cell-mediated bone regeneration

DLP-based bioprinting of void-forming hydrogels for enhanced stem-cell-mediated bone regeneration

  • Mater Today Bio. 2022 Nov 5;17:100487. doi: 10.1016/j.mtbio.2022.100487.
Jie Tao 1,  Shunyao Zhu 1,  Xueyuan Liao 1,  Yu Wang 1,  Nazi Zhou 1,  Zhan Li 2,  Haoyuan Wan 1,  Yaping Tang 1,  Sen Yang 1,  Ting Du 3,  Yang Yang 1,  Jinlin Song 4,  Rui Liu 1
Affiliations

Affiliations

  • 1 Department of Stomatology, Daping Hospital, Army Medical University (The Third Military Medical University), Chongqing, 400042, China.
  • 2 Department of Stem Cell and Regenerative Medicine, State Key Laboratory of Trauma, Burn and Combined Injury, Daping Hospital, Army Medical University, Chongqing, 400042, China.
  • 3 Non-coding RNA and Drug Discovery Key Laboratory of Sichuan Province, Chengdu Medical College, Chengdu, Sichuan, China.
  • 4 College of Stomatology, Chongqing Medical University, Chongqing, China.
PMID: 36388461 DOI: 10.1016/j.mtbio.2022.100487
Abstract

The integration of 3D bioprinting and stem cells is of great promise in facilitating the reconstruction of cranial defects. However, the effectiveness of the scaffolds has been hampered by the limited cell behavior and functions. Herein, a therapeutic cell-laden hydrogel for bone regeneration is therefore developed through the design of a void-forming hydrogel. This hydrogel is prepared by digital LIGHT processing (DLP)-based bioprinting of the bone marrow stem cells (BMSCs) mixed with gelatin methacrylate (GelMA)/dextran emulsion. The 3D-bioprinted hydrogel can not only promote the proliferation, migration, and spreading of the encapsulated BMSCs, but also stimulate the YAP signal pathway, thus leading to the enhanced osteogenic differentiation of BMSCs. In addition, the in vivo therapeutic assessments reveal that the void-forming hydrogel shows great potential for BMSCs delivery and can significantly promote bone regeneration. These findings suggest that the unique 3D-bioprinted void-forming hydrogels are promising candidates for applications in bone regeneration.

Keywords

3D bioprinting; Bone marrow stem cells; Bone regeneration; Macroporous hydrogel; Void-forming.

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