2. Academic Validation
  3. Transplantation of neural stem progenitor cells from different sources for severe spinal cord injury repair in rat

Transplantation of neural stem progenitor cells from different sources for severe spinal cord injury repair in rat

  • Bioact Mater. 2022 Nov 23:23:300-313. doi: 10.1016/j.bioactmat.2022.11.008.
Bai Xu 1 2,  Man Yin 1,  Yaming Yang 3,  Yunlong Zou 1 4,  Wenbin Liu 1,  Lianyong Qiao 1,  Jixiang Zhang 1,  Zhan Wang 1,  Yayu Wu 1,  He Shen 5,  Minghan Sun 1,  Weiyuan Liu 1,  Weiwei Xue 1,  Yongheng Fan 1,  Qi Zhang 1,  Bing Chen 1,  Xianming Wu 1,  Ya Shi 1,  Falong Lu 1,  Yannan Zhao 1,  Zhifeng Xiao 1,  Jianwu Dai 1 5
Affiliations

Affiliations

  • 1 State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
  • 2 Wuxi School of Medicine, Jiangnan University, Wuxi, 214122, China.
  • 3 Peking University Health Science Center, Beijing, 100191, China.
  • 4 China-Japan Union Hospital of Jilin University, Changchun, 130033, China.
  • 5 Division of Nanobiomedicine, Suzhou Institute of NanoTech and NanoBionics, Chinese Academy of Sciences, Suzhou, 215123, China.
PMID: 36439085 DOI: 10.1016/j.bioactmat.2022.11.008
Abstract

Neural stem progenitor cell (NSPC) transplantation has been regarded as a promising therapeutic method for spinal cord injury (SCI) repair. However, different NSPCs may have different therapeutic effects, and it is therefore important to identify the optimal NSPC type. In our study, we compared the transcriptomes of human fetal brain-derived NSPCs (BNSPCs), spinal cord-derived NSPCs (SCNSPCs) and H9 embryonic stem-cell derived NSPCs (H9-NSPCs) in vitro and subsequently we transplanted each NSPC type on a Collagen scaffold into a T8-9 complete SCI rat model in vivo. In vitro data showed that SCNSPCs had more highly expressed genes involved in nerve-related functions than the Other two cell types. In vivo, compared with BNSPCs and H9-NSPCs, SCNSPCs exhibited the best therapeutic effects; in fact, SCNSPCs facilitated electrophysiological and hindlimb functional recovery. This study demonstrates that SCNSPCs may be an appropriate candidate cell type for SCI repair, which is of great clinical significance.

Keywords

Brain-derived NSPCs; Collagen scaffolds; H9 embryonic stem cell-derived NSPCs; Spinal cord injury; Spinal cord-derived NSPCs.

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