1. PROTAC
    Metabolic Enzyme/Protease
    Immunology/Inflammation
    Anti-infection
  2. Molecular Glues
    Phosphatase
    Complement System
    Antibiotic
  3. Cyclosporin A

Cyclosporin A  (Synonyms: Cyclosporine A; Ciclosporin A; CsA)

Cat. No.: HY-B0579 Purity: 99.85%
COA Handling Instructions

Cyclosporine A est un immunosuppresseur qui se lie à la cyclophiline et inhibe l'activité phosphatase de la calcineurine avec un IC50 de 5 nM. Cyclosporine A inhibe également l'adhésion CD11a/CD18.

Cyclosporin A ist ein Immunsuppressivum, das an das Cyclophilin bindet und die Phosphataseaktivität von Calcineurin mit einem IC50 von 5 nM hemmt. Cyclosporin A hemmt auch die CD11a/CD18-Adhäsion.

Cyclosporin A (Cyclosporine A) is an immunosuppressant which binds to the cyclophilin and inhibits phosphatase activity of calcineurin with an IC50 of 5 nM. Cyclosporin A also inhibits CD11a/CD18 adhesion.

For research use only. We do not sell to patients.

Cyclosporin A Chemical Structure

Cyclosporin A Chemical Structure

CAS No. : 59865-13-3

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Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
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Estimated Time of Arrival: December 31
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100 mg USD 60 In-stock
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Customer Review

Based on 84 publication(s) in Google Scholar

Other Forms of Cyclosporin A:

Top Publications Citing Use of Products

70 Publications Citing Use of MCE Cyclosporin A

Proliferation Assay
WB
IF

    Cyclosporin A purchased from MCE. Usage Cited in: Cell Death Dis. 2018 May 22;9(6):598.  [Abstract]

    A549 cells are pretreated with 5 µM Cyclosporin A (CSA) for 2 h, followed by treatment with 80 μM Hirsutine for 24 h. equal amount of lysates are subjected to immunoprecipitation using anti-ANT1 antibody, the associated CypD is determined using immunoblotting.

    Cyclosporin A purchased from MCE. Usage Cited in: Nat Commun. 2021 May 18;12(1):2915.  [Abstract]

    Immunoblot analysis of Cytochrome c from cytosolic extracts and mitochondria from indicated cells pre-treated with DMSO or Cyclosporine A (50 μM) for 1 h, followed with PFOS treatment (150 μM, 6 h).

    Cyclosporin A purchased from MCE. Usage Cited in: Proc Natl Acad Sci U S A. 2021 Jan 12;118(2):e2009539118.  [Abstract]

    Calcineurin activities of the PCE after FK506 or Cyclosporine A (CsA; 4 µM) treatment Zebrafish.

    Cyclosporin A purchased from MCE. Usage Cited in: Exp Mol Med. 2022 Feb;54(2):129-142.  [Abstract]

    Representative western blotting images of cGAS and STING in the Cyclosporine (CsA; 10 μM; for 24 h)- or DMSO-treated NP cells.

    Cyclosporin A purchased from MCE. Usage Cited in: Exp Mol Med. 2022 Feb;54(2):129-142.  [Abstract]

    Representative immunofluorescence images of STING with different degrees of polymerization in the Cyclosporine (CsA; 10 μM; for 24 h)- or DMSO-treated NP cells.

    Cyclosporin A purchased from MCE. Usage Cited in: Exp Mol Med. 2022 Feb;54(2):129-142.  [Abstract]

    Representative western blotting images of NLRP3, ASC, pro-CASP-1, cleaved CASP-1, GSDMD, and cleaved GSDMD in the (CsA; 10 μM; for 24 h)- or DMSO-treated NP cells.

    Cyclosporin A purchased from MCE. Usage Cited in: Redox Biol. 2022 Aug;54:102355.

    Cells are treated with or without Cyclosporine A (CsA; 2 μM) for 4 h in presence of NRC-03 (45 μg/mL). Cell viability determined by CCK-8.

    Cyclosporin A purchased from MCE. Usage Cited in: Redox Biol. 2022 Aug;54:102355.

    Cells are treated with or without Cyclosporine A (CsA; 2 μM) for 4 h in presence of NRC-03 (45 μg/mL). TUNEL immunofluorescence staining and quantification of TUNEL positive cells.

    Cyclosporin A purchased from MCE. Usage Cited in: Cell Rep. 2021 Nov 30;37(9):110069.  [Abstract]

    Colony formation of CRC cells treated with Cyclosporine A (CsA; 2.5-5 μM) in combination with 5-FU or OXA for 48 h.

    Cyclosporin A purchased from MCE. Usage Cited in: Cell Rep. 2021 Nov 30;37(9):110069.  [Abstract]

    Immunoblotting analysis of indicated apoptotic markers expression in CRC cells treated with Cyclosporine A (CsA; 2.5-5 μM) in combination with 5-FU or OXA.

    Cyclosporin A purchased from MCE. Usage Cited in: Aging Dis. 2022 Oct 1;13(5):1546-1561.  [Abstract]

    Additionally, similar to P-gp siRNA silence, pharmacological inhibition of P-gp with Cyclosporine A (CsA; 22 mg/kg) significantly attenuates P-gp expression levels in ischemic brain as compared to vehicle treatment.

    Cyclosporin A purchased from MCE. Usage Cited in: Cell Death Dis. 2021 Apr 1;12(4):336.  [Abstract]

    Immunofluorescence staining of subcellular localization of NFATC1 in RANK overexpression and control cells with or without treatment of 10 μg/mL Cyclosporin A (CsA).

    Cyclosporin A purchased from MCE. Usage Cited in: Cell Death Dis. 2021 Apr 20;12(5):413.  [Abstract]

    Inhibition of NR-induced increase of co-localized puncta (LC3 and TOM20) after 1 μMCyclosporin A (CsA) pretreatment.

    Cyclosporin A purchased from MCE. Usage Cited in: Br J Pharmacol. 2021 Jun;178(11):2305-2323.  [Abstract]

    Smooth muscle cells are treated cyclosporin A (CsA, 1 μM) or PDTC (10 μM) for 24 h before measuring the activity of PPARγ2 promoter.
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Cyclosporin A (Cyclosporine A) is an immunosuppressant which binds to the cyclophilin and inhibits phosphatase activity of calcineurin with an IC50 of 5 nM[3]. Cyclosporin A also inhibits CD11a/CD18 adhesion[8].

    IC50 & Target

    IC50: 7 nM (calcineurin)

    In Vitro

    Cyclosporin A is able to bind with the cyclophilin in T cells[1]. Cyclosporin A works by forming a Cyclophilin-Cyclosporin A complex to inhibit calcineurin[2]. Cyclosporin A inhibits calcineurin in stimulated cells with an IC50 value of 7 nM[3]. Cyclosporin A suppresses the nuclear translocation of NF-AT[4]. Cyclosporin A shows an effect on mitochondria via preventing the MTP from opening with an IC50 of 39 nM[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Cyclosporin A has immunosuppressive activity, and is active via parenteral and p.o. administration in mice, rat and guinea pigs[6]. Cyclosporin A can be used in organ transplantation to prevent rejection[7].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Clinical Trial
    Molecular Weight

    1202.61

    Formula

    C62H111N11O12

    CAS No.
    SMILES

    C/C=C/C[[email protected]]([[email protected]@H](O)[[email protected]@]1(N(C)C([[email protected]]([H])(C(C)C)N(C)C([[email protected]](CC(C)C)N(C)C([[email protected]]([H])(CC(C)C)N(C)C([[email protected]@H](C)NC([[email protected]](C)NC([[email protected]@H](N(C([[email protected]@]([H])(NC([[email protected]@H](N(C(CN(C([[email protected]@H](NC1=O)CC)=O)C)=O)C)CC(C)C)=O)C(C)C)=O)C)CC(C)C)=O)=O)=O)=O)=O)=O)[H])C

    Structure Classification
    Source

    the fungus Beauveria nivea.

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 62.5 mg/mL (51.97 mM; Need ultrasonic)

    Ethanol : 50 mg/mL (41.58 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.8315 mL 4.1576 mL 8.3152 mL
    5 mM 0.1663 mL 0.8315 mL 1.6630 mL
    10 mM 0.0832 mL 0.4158 mL 0.8315 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  corn oil

      Solubility: 20 mg/mL (16.63 mM); Clear solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  5% DMSO    40% PEG300    5% Tween-80    50% saline

      Solubility: 2.62 mg/mL (2.18 mM); Suspended solution; Need ultrasonic

    • 3.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: 2.08 mg/mL (1.73 mM); Suspended solution; Need ultrasonic

    • 4.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 2.08 mg/mL (1.73 mM); Suspended solution; Need ultrasonic

    • 5.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (1.73 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.85%

    References
    Cell Assay
    [3]

    Immunosuppressive agents are dissolved in ethanol at concentrations 1000-fold more than the concentration desired for cell treatments. Cells (106) are suspended in 1 mL of complete medium in microcentrifuge tubes; 1 μL of ethanol or of the ethanolic solution of Cyclosporin A is added, and the cells are incubated at 37°C for 1 hr. Cells are washed twice with 1 mL of PBS on ice and lysed in 50 μL of hypotonic buffer containing 50 mM Tris (pH 7.5); 0.1 mM EGTA; 1 mM EDTA; 0.5 mM dithiothreitol; and 50 μg of phenylmethylsulfonyl fluoride, 50 μg of soybean trypsin inhibitor, 5 μg of leupeptin, and 5 μg of Kiker 52G per mL. Lysates are subjected to three cycles of freezing in liquid nitrogen followed by thawing at 30°C and then are centrifuged at 4°C for 10 min at 12,000×g.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [6]

    Rats are immunized on day 0 i.p. with 0 5 mL and mice i.v. with 0 2 mL of a 10% suspension of washed sheep erythrocytes (SE). To elicit a secondary response, mice are boosted 8-11 weeks after the primary immunization with an i.v. injection of 0-2 mL of 0 25% washed SE (107 cells). For prolonged treatment the animals receive on the average 45 mg/kg cyclosporin A daily in the food during 13 weeks. These rats are immunized 5 days before killing.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
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    Cat. No.:
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