2. Academic Validation
  3. ONE-seq: epitranscriptome and gene-specific profiling of NAD-capped RNA

ONE-seq: epitranscriptome and gene-specific profiling of NAD-capped RNA

  • Nucleic Acids Res. 2022 Dec 7;gkac1136. doi: 10.1093/nar/gkac1136.
Kongyan Niu 1 2,  Jinyang Zhang 1 2,  Shuwen Ge 1 2,  Dean Li 1 2,  Kunfeng Sun 1 2,  Yingnan You 1 2,  Jiaqian Qiu 1 2,  Kun Wang 1 2,  Xueting Wang 1 2,  Rui Liu 3,  Yandong Liu 4,  Bing Li 5,  Zheng-Jiang Zhu 1 6,  Lefeng Qu 4,  Hong Jiang 1,  Nan Liu 1 6
Affiliations

Affiliations

  • 1 Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 100 Hai Ke Rd., Pudong, Shanghai 201210, China.
  • 2 University of Chinese Academy of Sciences, Beijing 100049, China.
  • 3 Singlera Genomics, 500 Fu Rong Hua Rd., Pudong, Shanghai 201204, China.
  • 4 Department of Vascular and Endovascular Surgery, Chang Zheng Hospital, Naval Medical University, Shanghai 200003, China.
  • 5 IH Bluex Technology, 58 Xiang Cheng Road, Shanghai 200122, China.
  • 6 Shanghai Key Laboratory of Aging Studies, 100 Hai Ke Rd., Pudong, Shanghai 201210, China.
PMID: 36477375 DOI: 10.1093/nar/gkac1136
Abstract

The hub metabolite, nicotinamide adenine dinucleotide (NAD), can be used as an initiating nucleotide in RNA synthesis to result in NAD-capped RNAs (NAD-RNA). Since NAD has been heightened as one of the most essential modulators in aging and various age-related diseases, its attachment to RNA might indicate a yet-to-be discovered mechanism that impacts adult life-course. However, the unknown identity of NAD-linked RNAs in adult and aging tissues has hindered functional studies. Here, we introduce ONE-seq method to identify the RNA transcripts that contain NAD cap. ONE-seq has been optimized to use only one-step chemo-enzymatic biotinylation, followed by streptavidin capture and the nudix phosphohydrolase NudC-catalyzed elution, to specifically recover NAD-capped RNAs for epitranscriptome and gene-specific analyses. Using ONE-seq, we discover more than a thousand of previously unknown NAD-RNAs in the mouse liver and reveal epitranscriptome-wide dynamics of NAD-RNAs with age. ONE-seq empowers the identification of NAD-capped RNAs that are responsive to distinct physiological states, facilitating functional investigation into this modification.

Figures
Products