2. Academic Validation
  3. Viscoelastic Liquid Matrix with Faster Bulk Relaxation Time Reinforces the Cell Cycle Arrest Induction of the Breast Cancer Cells via Oxidative Stress

Viscoelastic Liquid Matrix with Faster Bulk Relaxation Time Reinforces the Cell Cycle Arrest Induction of the Breast Cancer Cells via Oxidative Stress

  • Int J Mol Sci. 2022 Nov 24;23(23):14637. doi: 10.3390/ijms232314637.
Mazaya Najmina 1 2,  Mitsuhiro Ebara 1 2 3,  Takahito Ohmura 4,  Koichiro Uto 1
Affiliations

Affiliations

  • 1 Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba 305-0044, Japan.
  • 2 Graduate School of Science and Engineering, University of Tsukuba, 1-1 Tennodai, Tsukuba 305-8577, Japan.
  • 3 Graduate School of Industrial Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 162-8601, Japan.
  • 4 Research Center for Structural Materials, National Institute for Materials Science, 1-2-1 Sengen, Tsukuba 305-0047, Japan.
PMID: 36498966 DOI: 10.3390/ijms232314637
Abstract

The reactivating of disseminated dormant breast Cancer cells in a soft viscoelastic matrix is mostly correlated with metastasis. Metastasis occurs due to rapid stress relaxation owing to matrix remodeling. Here, we demonstrate the possibility of promoting the permanent cell cycle arrest of breast Cancer cells on a viscoelastic liquid substrate. By controlling the molecular weight of the hydrophobic molten polymer, poly(ε-caprolactone-co-D,L-lactide) within 35-63 g/mol, this study highlights that MCF7 cells can sense a 1000 times narrower relaxation time range (80-290 ms) compared to Other studies by using a crosslinked hydrogel system. We propose that the rapid bulk relaxation response of the substrate promotes more Reactive Oxygen Species generation in the formed semi-3D multicellular aggregates of breast Cancer cells. Our finding sheds light on the potential role of bulk stress relaxation in a viscous-dominant viscoelastic matrix in controlling the cell cycle arrest depth of breast Cancer cells.

Keywords

breast cancer; material-induced senescence; multicellular aggregates; oxidative stress; stress relaxation.

Figures
Products